| pubmed-article:19626615 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0220847 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0023884 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C1623038 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0599161 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0205210 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C0205088 | lld:lifeskim |
| pubmed-article:19626615 | lifeskim:mentions | umls-concept:C2347946 | lld:lifeskim |
| pubmed-article:19626615 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:19626615 | pubmed:dateCreated | 2009-7-28 | lld:pubmed |
| pubmed-article:19626615 | pubmed:abstractText | HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand-specific HCV real-time RT-PCR assays were developed and applied to livers explanted because of end-stage cirrhosis. The assays have broad ranges of determination and a high reproducibility and accuracy. Analysis of five different samples showed an even distribution of HCV genomes in four livers. Hepatic concentrations of positive (PS)- and negative (NS)-strand RNA did correlate with each other, with PS/NS ratios ranging between 3 and 340. Hepatic concentrations of HCV-PS or -NS RNA did not correlate with serum HCV-RNA levels or with genotypes. A high HCV envelope-2 protein expression correlated with a low NS concentration. HCV-PS and -NS levels, E2 protein expression and genotype did not correlate with biochemical tests or with histological changes in the explanted liver, but the ratio NS/PS, a marker of viral replication, correlated with the severity of the recurrent post-transplant hepatitis caused by HCV. This suggests the existence of an extra-hepatic location of HCV with comparable viral replication rate being responsible for the infection of the newly transplanted liver. | lld:pubmed |
| pubmed-article:19626615 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19626615 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19626615 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19626615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19626615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19626615 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19626615 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:19626615 | pubmed:issn | 1096-9071 | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:LinLanL | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:FeveryJohanJ | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:RoskamsTaniaT | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:LibbrechtLoui... | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:VerslypeChris... | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:van PeltJosJ | lld:pubmed |
| pubmed-article:19626615 | pubmed:author | pubmed-author:VerbeeckJanni... | lld:pubmed |
| pubmed-article:19626615 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19626615 | pubmed:volume | 81 | lld:pubmed |
| pubmed-article:19626615 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19626615 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19626615 | pubmed:pagination | 1569-75 | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:meshHeading | pubmed-meshheading:19626615... | lld:pubmed |
| pubmed-article:19626615 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19626615 | pubmed:articleTitle | Quantitation of replication of the HCV genome in human livers with end-stage cirrhosis by strand-specific real-time RT-PCR assays: methods and clinical relevance. | lld:pubmed |
| pubmed-article:19626615 | pubmed:affiliation | Department of Hepatology, University Hospitals, Leuven, Belgium. | lld:pubmed |
| pubmed-article:19626615 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19626615 | lld:pubmed |
