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pubmed-article:19570806pubmed:abstractTextThe clustering of expressed sequence tags (ESTs) is a crucial step in many sequence analysis studies that require a high level of redundancy. Chimeric sequences, while uncommon, can make achieving the optimal EST clustering a challenge. Single-linkage algorithms are particularly vulnerable to the effects of chimeras. To avoid chimera-facilitated erroneous merges, researchers using single-linkage algorithms are forced to use stringent sequence-similarity thresholds. Such thresholds reduce the sensitivity of the clustering algorithm.lld:pubmed
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pubmed-article:19570806pubmed:authorpubmed-author:StoneGlennGlld:pubmed
pubmed-article:19570806pubmed:authorpubmed-author:BraggLauren...lld:pubmed
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pubmed-article:19570806pubmed:articleTitlek-link EST clustering: evaluating error introduced by chimeric sequences under different degrees of linkage.lld:pubmed
pubmed-article:19570806pubmed:affiliationCSIRO Mathematical and Information Sciences, North Ryde, NSW, Australia. lauren.bragg@csiro.aulld:pubmed
pubmed-article:19570806pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19570806pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed