| pubmed-article:19549857 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C1123023 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C1095830 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0282641 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0443315 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0031560 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0021102 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0870883 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0544341 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:19549857 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:19549857 | pubmed:issue | 26 | lld:pubmed |
| pubmed-article:19549857 | pubmed:dateCreated | 2009-7-1 | lld:pubmed |
| pubmed-article:19549857 | pubmed:abstractText | Adjustable control of therapeutic transgenes in engineered cell implants after transdermal and topical delivery of nontoxic trigger molecules would increase convenience, patient compliance, and elimination of hepatic first-pass effect in future therapies. Pseudomonas putida DOT-T1E has evolved the flavonoid-triggered TtgR operon, which controls expression of a multisubstrate-specific efflux pump (TtgABC) to resist plant-derived defense metabolites in its rhizosphere habitat. Taking advantage of the TtgR operon, we have engineered a hybrid P. putida-mammalian genetic unit responsive to phloretin. This flavonoid is contained in apples, and, as such, or as dietary supplement, regularly consumed by humans. The engineered mammalian phloretin-adjustable control element (PEACE) enabled adjustable and reversible transgene expression in different mammalian cell lines and primary cells. Due to the short half-life of phloretin in culture, PEACE could also be used to program expression of difficult-to-produce protein therapeutics during standard bioreactor operation. When formulated in skin lotions and applied to the skin of mice harboring transgenic cell implants, phloretin was able to fine-tune target genes and adjust heterologous protein levels in the bloodstream of treated mice. PEACE-controlled target gene expression could foster advances in biopharmaceutical manufacturing as well as gene- and cell-based therapies. | lld:pubmed |
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| pubmed-article:19549857 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19549857 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19549857 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19549857 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19549857 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19549857 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19549857 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19549857 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:19549857 | pubmed:author | pubmed-author:FusseneggerMa... | lld:pubmed |
| pubmed-article:19549857 | pubmed:author | pubmed-author:WeberWilfried... | lld:pubmed |
| pubmed-article:19549857 | pubmed:author | pubmed-author:El-BabaMarie... | lld:pubmed |
| pubmed-article:19549857 | pubmed:author | pubmed-author:KemmerChristi... | lld:pubmed |
| pubmed-article:19549857 | pubmed:author | pubmed-author:GitzingerMarc... | lld:pubmed |
| pubmed-article:19549857 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19549857 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:19549857 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:19549857 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19549857 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19549857 | pubmed:pagination | 10638-43 | lld:pubmed |
| pubmed-article:19549857 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
