| pubmed-article:19536353 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19536353 | lifeskim:mentions | umls-concept:C0684249 | lld:lifeskim |
| pubmed-article:19536353 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:19536353 | lifeskim:mentions | umls-concept:C0080032 | lld:lifeskim |
| pubmed-article:19536353 | lifeskim:mentions | umls-concept:C0019409 | lld:lifeskim |
| pubmed-article:19536353 | lifeskim:mentions | umls-concept:C1517564 | lld:lifeskim |
| pubmed-article:19536353 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:19536353 | pubmed:dateCreated | 2009-6-18 | lld:pubmed |
| pubmed-article:19536353 | pubmed:abstractText | Malignant Pleural Effusions (MPE) may be useful as a model to study hierarchical progression of cancer and/or intratumoral heterogeneity. To strengthen the rationale for developing the MPE-model for these purposes, we set out to find evidence for the presence of cancer stem cells (CSC) in MPE and demonstrate an ability to sustain intratumoral heterogeneity in MPE-primary cultures. Our studies show that candidate lung CSC-expression signatures (PTEN, OCT4, hTERT, Bmi1, EZH2 and SUZ12) are evident in cell pellets isolated from MPE, and MPE-cytopathology also labels candidate-CSC (CD44, cMET, MDR-1, ALDH) subpopulations. Moreover, in primary cultures that use MPE as the source of both tumor cells and the tumor microenvironment (TME), candidate CSC are maintained over time. This allows us to live-sort candidate CSC-fractions from the MPE-tumor mix on the basis of surface markers (CD44, c-MET, uPAR, MDR-1) or differences in xenobiotic metabolism (ALDH). Thus, MPE-primary cultures provide an avenue to extract candidate CSC populations from individual (isogenic) MPE-tumors. This will allow us to test whether these cells can be discriminated in functional bioassays. Tumor heterogeneity in MPE-primary cultures is evidenced by variable immunolabeling, differences in colony-morphology, and differences in proliferation rates of cell subpopulations. Collectively, these data justify the ongoing development of the MPE-model for the investigation of intratumoral heterogeneity, tumor-TME interactions, and phenotypic validation of candidate lung CSC, in addition to providing direction for the pre-clinical development of rational therapeutics. | lld:pubmed |
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| pubmed-article:19536353 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19536353 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19536353 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19536353 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19536353 | pubmed:issn | 1932-6203 | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:SharmaSherven... | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:HuangMinM | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:HuangGeG | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:BatraRaj KRK | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:BasakSaroj... | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:VeenaMysore... | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:OhScottS | lld:pubmed |
| pubmed-article:19536353 | pubmed:author | pubmed-author:SrivatsanEriE | lld:pubmed |
| pubmed-article:19536353 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19536353 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:19536353 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19536353 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19536353 | pubmed:pagination | e5884 | lld:pubmed |
| pubmed-article:19536353 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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