| pubmed-article:19531770 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19531770 | lifeskim:mentions | umls-concept:C0026764 | lld:lifeskim |
| pubmed-article:19531770 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:19531770 | lifeskim:mentions | umls-concept:C0376452 | lld:lifeskim |
| pubmed-article:19531770 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:19531770 | lifeskim:mentions | umls-concept:C1515021 | lld:lifeskim |
| pubmed-article:19531770 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:19531770 | pubmed:dateCreated | 2009-7-30 | lld:pubmed |
| pubmed-article:19531770 | pubmed:abstractText | Multiple myeloma (MM) is characterized by a wide spectrum of genetic changes. Global hypomethylation of repetitive genomic sequences such as long interspersed nuclear element 1 (LINE-1), Alu and satellite alpha (SAT-alpha) sequences has been associated with chromosomal instability in cancer. Methylation status of repetitive elements in MM has never been investigated. In the present study, we used a quantitative bisulfite-polymerase chain reaction pyrosequencing method to evaluate the methylation patterns of LINE-1, Alu and SAT-alpha in 23 human myeloma cell lines (HMCLs) and purified bone marrow plasma cells from 53 newly diagnosed MM patients representative of different molecular subtypes, 7 plasma cell leukemias (PCLs) and 11 healthy controls. MMs showed a decrease of Alu [median: 21.1 %5-methylated cytosine (%5mC)], LINE-1 (70.0%5mC) and SAT-alpha (77.9%5mC) methylation levels compared with controls (25.2, 79.5and 89.5%5mC, respectively). Methylation levels were lower in PCLs and HMCLs compared with MMs (16.7 and 14.8%5mC for Alu, 45.5 and 42.4%5mC for LINE-1 and 33.3 and 43.3%5mC for SAT-alpha, respectively). Notably, LINE-1 and SAT-alpha methylation was significantly lower in the non-hyperdiploid versus hyperdiploid MMs (P = 0.01 and 0.02, respectively), whereas Alu and SAT-alpha methylation was significantly lower in MMs with t(4;14) (P = 0.02 and 0.004, respectively). Finally, we correlated methylation patterns with DNA methyltransferases (DNMTs) messenger RNA levels showing in particular a progressive and significant increase of DNMT1 expression from controls to MMs, PCLs and HMCLs (P < 0.001). Our results indicate that global hypomethylation of repetitive elements is significantly associated with tumor progression in MM and may contribute toward a more extensive stratification of the disease. | lld:pubmed |
| pubmed-article:19531770 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19531770 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19531770 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19531770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19531770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19531770 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19531770 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:19531770 | pubmed:issn | 1460-2180 | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:MoscaLauraL | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:BaccarelliAnd... | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:BertazziPier... | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:DeliliersGior... | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:NeriAntoninoA | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:RonchettiDome... | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:FabrisSoniaS | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:BollatiValent... | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:PegoraroValer... | lld:pubmed |
| pubmed-article:19531770 | pubmed:author | pubmed-author:MottaValeriaV | lld:pubmed |
| pubmed-article:19531770 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19531770 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:19531770 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19531770 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19531770 | pubmed:pagination | 1330-5 | lld:pubmed |
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| pubmed-article:19531770 | pubmed:meshHeading | pubmed-meshheading:19531770... | lld:pubmed |
| pubmed-article:19531770 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19531770 | pubmed:articleTitle | Differential repetitive DNA methylation in multiple myeloma molecular subgroups. | lld:pubmed |
| pubmed-article:19531770 | pubmed:affiliation | Center of Molecular and Genetic Epidemiology, EPOCA, Epidemiology Research Center, Università degli Studi di Milano and Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, 20122 Milan, Italy. | lld:pubmed |
| pubmed-article:19531770 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19531770 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:1786 | entrezgene:pubmed | pubmed-article:19531770 | lld:entrezgene |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19531770 | lld:pubmed |
