| pubmed-article:19500086 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19500086 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19500086 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:19500086 | lifeskim:mentions | umls-concept:C0966897 | lld:lifeskim |
| pubmed-article:19500086 | lifeskim:mentions | umls-concept:C0034580 | lld:lifeskim |
| pubmed-article:19500086 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19500086 | pubmed:dateCreated | 2009-7-23 | lld:pubmed |
| pubmed-article:19500086 | pubmed:abstractText | The hepatic peptide hormone hepcidin plays a central role in body iron metabolism. Despite its promise as a biomarker, the availability of high-sensitive hepcidin assays is still limited. We developed and validated a RadioImmunoAssay (RIA) to measure hepcidin quantitatively in human serum. This assay exhibited a very low detection limit (0.02 microg/l), low imprecision (coefficient of variation-range 4.4-6.2%) and good linearity and recovery (range: 81-105%). Hepcidin levels of samples of controls and patients with iron deficiency and inflammation showed an excellent correlation with our previously described quantitative time-of-flight mass spectrometry assay (range 2.5-266.8 microg/l, r = 0.92, P < 0.0001). The RIA detected: (i) differences in mean hepcidin levels between men (n = 29) and women (n = 35), (ii) differences between individuals of different HFE-genotypes (n = 60) and (iii) daily increases in hepcidin levels (n = 64). The assay (i) is easy to perform and many samples can be processed within one assay-run, (ii) shows accurate, reproducible and high-sensitive measurements and (iii) is anticipated to be particularly useful to study the effects of pathological and physiological stimuli on hepcidin levels in the lower range. | lld:pubmed |
| pubmed-article:19500086 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19500086 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19500086 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19500086 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19500086 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19500086 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19500086 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19500086 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:19500086 | pubmed:issn | 1365-2141 | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:TjalsmaHarold... | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:SwinkelsDorin... | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:den... | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:SweepFred G... | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:Grebenchtchik... | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:Geurts-Moespo... | lld:pubmed |
| pubmed-article:19500086 | pubmed:author | pubmed-author:KrootJoyce... | lld:pubmed |
| pubmed-article:19500086 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19500086 | pubmed:volume | 146 | lld:pubmed |
| pubmed-article:19500086 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19500086 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19500086 | pubmed:pagination | 317-25 | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
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| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
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| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:meshHeading | pubmed-meshheading:19500086... | lld:pubmed |
| pubmed-article:19500086 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19500086 | pubmed:articleTitle | High-sensitive radioimmunoassay for human serum hepcidin. | lld:pubmed |
| pubmed-article:19500086 | pubmed:affiliation | Department of Chemical Endocrinology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. | lld:pubmed |
| pubmed-article:19500086 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19500086 | pubmed:publicationType | Validation Studies | lld:pubmed |
| entrez-gene:3077 | entrezgene:pubmed | pubmed-article:19500086 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19500086 | lld:entrezgene |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19500086 | lld:pubmed |
