| pubmed-article:19479780 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19479780 | lifeskim:mentions | umls-concept:C0524637 | lld:lifeskim |
| pubmed-article:19479780 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:19479780 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:19479780 | lifeskim:mentions | umls-concept:C1705542 | lld:lifeskim |
| pubmed-article:19479780 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:19479780 | pubmed:dateCreated | 2009-6-3 | lld:pubmed |
| pubmed-article:19479780 | pubmed:abstractText | A novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (APBA) as a functional monomer. The produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. Results confirmed that the synthesized BSA-MIP (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. The produced BSA-MIP featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. Non-specific binding was reduced to almost zero in a BSA/BHb competitive binding event. An excellent HPLC profile of template recognition was found for BSA-MIP, even under harsh mobile phase conditions. In the present work, the adopted trapped-template-release method permits recovery of bound BSA [1]. The strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments. | lld:pubmed |
| pubmed-article:19479780 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19479780 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19479780 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19479780 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19479780 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19479780 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19479780 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19479780 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19479780 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19479780 | pubmed:issn | 1615-9314 | lld:pubmed |
| pubmed-article:19479780 | pubmed:author | pubmed-author:ZhangYukuiY | lld:pubmed |
| pubmed-article:19479780 | pubmed:author | pubmed-author:HeXiwenX | lld:pubmed |
| pubmed-article:19479780 | pubmed:author | pubmed-author:ChenLangxingL | lld:pubmed |
| pubmed-article:19479780 | pubmed:author | pubmed-author:WangHuafangH | lld:pubmed |
| pubmed-article:19479780 | pubmed:author | pubmed-author:HeYunhuaY | lld:pubmed |
| pubmed-article:19479780 | pubmed:author | pubmed-author:LiWenyouW | lld:pubmed |
| pubmed-article:19479780 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19479780 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:19479780 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19479780 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19479780 | pubmed:pagination | 1981-6 | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:meshHeading | pubmed-meshheading:19479780... | lld:pubmed |
| pubmed-article:19479780 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19479780 | pubmed:articleTitle | BSA-imprinted synthetic receptor for reversible template recognition. | lld:pubmed |
| pubmed-article:19479780 | pubmed:affiliation | Department of Chemistry, Nankai University, Tianjin, P.R. China. | lld:pubmed |
| pubmed-article:19479780 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19479780 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
