Linked Life Data

19479242

Source:http://linkedlifedata.com/resource/pubmed/id/19479242

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:19479242rdf:typepubmed:Citationlld:pubmed
pubmed-article:19479242lifeskim:mentionsumls-concept:C0086418lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C0225336lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C0031715lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C0239059lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C0392747lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C1533691lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C0205263lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C2828366lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C1705851lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C0443172lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C1555707lld:lifeskim
pubmed-article:19479242lifeskim:mentionsumls-concept:C2752151lld:lifeskim
pubmed-article:19479242pubmed:issue6lld:pubmed
pubmed-article:19479242pubmed:dateCreated2009-6-24lld:pubmed
pubmed-article:19479242pubmed:abstractTextPhosphorylation is the most widely studied posttranslational modification (PTM) and is an important regulatory mechanism used during cellular responses to external stimuli. The kinases and phosphatases that regulate protein phosphorylation are known to be affected in many human diseases. Cigarette smoking causes cardiovascular disease (CVD). Endothelial cells play a pivotal role in CVD initiation and development; however, there have been limited investigations of the specific signaling cascades and protein phosphorylations activated by cigarette smoke in endothelial cells. The purpose of this research was to better understand the differential protein phosphorylation in endothelial cells stimulated with extracts of cigarette smoke total particulate matter (CS-TPM) in vitro. Human microvascular endothelial cells were exposed in vitro to CS-TPM at concentrations that were shown to cause endothelial cell dysfunction. The phosphorylated proteins were isolated using phosphoprotein-specific chromatography, followed by enzymatic digestion and nano-flow capillary liquid chromatography (ncap-LC) coupled to high resolution mass spectrometry. This study putatively identified 94 proteins in human microvascular endothelial cells that were differentially bound to a phosphoprotein-specific chromatography column following exposure to CS-TPM suggesting differential phosphorylation. Pathway analysis has also been conducted and confirmations of several observations have been made using immunoaffinity-based techniques (e.g., Western blotting).lld:pubmed
pubmed-article:19479242pubmed:languageenglld:pubmed
pubmed-article:19479242pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19479242pubmed:citationSubsetIMlld:pubmed
pubmed-article:19479242pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19479242pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19479242pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19479242pubmed:statusMEDLINElld:pubmed
pubmed-article:19479242pubmed:monthJullld:pubmed
pubmed-article:19479242pubmed:issn1618-2650lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:FloraJason...lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:EdmistonJeffe...lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:ScianMariano...lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:SenguptaTapas...lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:LiGuoyaGlld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:LangstonTimot...lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:McKinneyWilli...lld:pubmed
pubmed-article:19479242pubmed:authorpubmed-author:RanaGaurav...lld:pubmed
pubmed-article:19479242pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19479242pubmed:volume394lld:pubmed
pubmed-article:19479242pubmed:ownerNLMlld:pubmed
pubmed-article:19479242pubmed:authorsCompleteYlld:pubmed
pubmed-article:19479242pubmed:pagination1609-20lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:meshHeadingpubmed-meshheading:19479242...lld:pubmed
pubmed-article:19479242pubmed:year2009lld:pubmed
pubmed-article:19479242pubmed:articleTitleCigarette smoke extract induced protein phosphorylation changes in human microvascular endothelial cells in vitro.lld:pubmed
pubmed-article:19479242pubmed:affiliationAltria Client Services, 601 East Leigh Street, Richmond VA, 23219, USA.lld:pubmed
pubmed-article:19479242pubmed:publicationTypeJournal Articlelld:pubmed