| pubmed-article:19456860 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19456860 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:19456860 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19456860 | lifeskim:mentions | umls-concept:C0215993 | lld:lifeskim |
| pubmed-article:19456860 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
| pubmed-article:19456860 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:19456860 | pubmed:dateCreated | 2009-8-3 | lld:pubmed |
| pubmed-article:19456860 | pubmed:abstractText | Macrophage-stimulating protein (MSP) is a plasma protein that circulates as a single-chain proform. It acquires biological activity after proteolytic cleavage at the Arg483-Val484 bond, a process in which serum and cell surface serine proteinases have been implicated. In this article, we report that hepatocyte growth factor activator (HGFA), a serum proteinase which activates hepatocyte growth factor in response to tissue injury, may have a critical role in the activation of pro-MSP. In vitro analysis has revealed that human HGFA efficiently cleaves human pro-MSP at the physiological activation site without further degradation, resulting in biologically active MSP, as measured by the chemotactic response and MSP-induced morphological change of peritoneal macrophages. The processing of pro-MSP by HGFA is 10-fold more efficient than processing by factor XIa. To search for a role of HGFA in pro-MSP activation, we analyzed the processing of mouse pro-MSP in sera from HGFA-knockout (HGFA(-/-)) mice. The proform of MSP was the predominant molecular form in the plasma of both wild-type and HGFA(-/-) mice. In wild-type sera, endogenous pro-MSP was progressively converted to the mature two-chain form during incubation at 37 degrees C. However, this conversion was significantly impaired in sera from HGFA(-/-) mice. The addition of recombinant HGFA to HGFA-deficient serum restored pro-MSP convertase activity in a dose-dependent manner, and a neutralizing antibody to HGFA significantly reduced the conversion of pro-MSP in wild-type serum. Moreover, initial infiltration of macrophages into the site of mechanical skin injury was delayed in HGFA(-/-) mice. We suggest that HGFA is a major serum activator of pro-MSP. | lld:pubmed |
| pubmed-article:19456860 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19456860 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19456860 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19456860 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19456860 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19456860 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19456860 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19456860 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19456860 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19456860 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:19456860 | pubmed:issn | 1742-4658 | lld:pubmed |
| pubmed-article:19456860 | pubmed:author | pubmed-author:KataokaHiroak... | lld:pubmed |
| pubmed-article:19456860 | pubmed:author | pubmed-author:BabaTakashiT | lld:pubmed |
| pubmed-article:19456860 | pubmed:author | pubmed-author:FukushimaTsuy... | lld:pubmed |
| pubmed-article:19456860 | pubmed:author | pubmed-author:KawaguchiMaki... | lld:pubmed |
| pubmed-article:19456860 | pubmed:author | pubmed-author:OrikawaHirosh... | lld:pubmed |
| pubmed-article:19456860 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19456860 | pubmed:volume | 276 | lld:pubmed |
| pubmed-article:19456860 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19456860 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19456860 | pubmed:pagination | 3481-90 | lld:pubmed |
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| pubmed-article:19456860 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19456860 | pubmed:articleTitle | Hepatocyte growth factor activator is a serum activator of single-chain precursor macrophage-stimulating protein. | lld:pubmed |
| pubmed-article:19456860 | pubmed:affiliation | Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki, Japan. | lld:pubmed |
| pubmed-article:19456860 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19456860 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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