| pubmed-article:19433111 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C0019166 | lld:lifeskim |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:19433111 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:19433111 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:19433111 | pubmed:dateCreated | 2009-6-29 | lld:pubmed |
| pubmed-article:19433111 | pubmed:abstractText | The core antigen of the hepatitis B virus (HBcAg) has been used widely as a diagnostic reagent for the identification of the viral infection. However, purification using the conventional sucrose density gradient ultracentrifugation is time consuming and costly. To overcome this, HBcAg particles displaying His-tag on their surface were constructed and produced in Escherichia coli. The recombinant His-tagged HBcAgs were purified using immobilized metal affinity chromatography. Transmission electron microscopy and enzyme-linked immunosorbent assay (ELISA) revealed that the displayed His-tag did not impair the formation of the core particles and the antigenicity of HBcAg. | lld:pubmed |
| pubmed-article:19433111 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19433111 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19433111 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19433111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19433111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19433111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19433111 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19433111 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:19433111 | pubmed:issn | 1879-0984 | lld:pubmed |
| pubmed-article:19433111 | pubmed:author | pubmed-author:TanWen... | lld:pubmed |
| pubmed-article:19433111 | pubmed:author | pubmed-author:OngSwee TinST | lld:pubmed |
| pubmed-article:19433111 | pubmed:author | pubmed-author:TeyBeng TiBT | lld:pubmed |
| pubmed-article:19433111 | pubmed:author | pubmed-author:NgMichelle... | lld:pubmed |
| pubmed-article:19433111 | pubmed:author | pubmed-author:YapWei BoonWB | lld:pubmed |
| pubmed-article:19433111 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19433111 | pubmed:volume | 160 | lld:pubmed |
| pubmed-article:19433111 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19433111 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19433111 | pubmed:pagination | 125-31 | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:meshHeading | pubmed-meshheading:19433111... | lld:pubmed |
| pubmed-article:19433111 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19433111 | pubmed:articleTitle | N-terminally His-tagged hepatitis B core antigens: construction, expression, purification and antigenicity. | lld:pubmed |
| pubmed-article:19433111 | pubmed:affiliation | Department of Microbiology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. | lld:pubmed |
| pubmed-article:19433111 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19433111 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
