| pubmed-article:1941504 | pubmed:abstractText | The influence of pineal gland function and of melatonin during pregnancy upon sexual maturation of female offspring in rats was examined. The following groups of Wistar rats were studied: (A) control, (B) melatonin treated (250 micrograms/100 g.b.w. per day) throughout pregnancy, and (c) pinealectomized (pin-x). Melatonin was injected subcutaneously 2 hr before lights off. All groups were housed in 12-hr L:12-hr D. The female offspring were observed until the onset of puberty, and blood samples were collected 5.5 hr after lights off under red light for melatonin and LH determinations by RIA. Ovary, pineal, and pituitary gland as well as body weights were recorded. The offspring of melatonin-treated rats showed later vaginal opening than did those of the other two groups (P less than 0.05 vs. control and P less than 0.01 vs. pin-x offspring), which was accompanied by a lower LH concentration, 0.42 + 0.05 ng LH/ml, showing statistically significant differences with the control levels [1.00 + 0.22 ng LH/ml (P less than 0.05)] and with the pin-x group [1.16 + 0.22 ng LH/ml (P less than 0.05)]. The percentage of rats in proestrus was higher in the offspring of pin-x rats (78.6%; P less than 0.01) compared to control offspring (30%) and offspring of melatonin-treated rats (11.8%). The concentrations of melatonin, 5.5 hr after darkness, were not significantly different among groups. No differences were observed in the body, ovarian, and pineal weights of the studied offspring. The pituitary weight was significantly lower (P less than 0.05) in the offspring of pin-x rats than in the other two groups.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
