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pubmed-article:1940612pubmed:abstractTextTo further define the ultrastructural events associated with the killing of Candida albicans by human neutrophils, four methods were used: (1) the periodate-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining of vicinal-glycol-containing complex carbohydrates; (2) the localization of thermostable immunodeterminants of the yeast cell wall, mannans or mannoproteins, using monospecific antibodies and a protein A-gold complex (monAb-gold); (3) the localization of mannose residues with concanavalin A labeled with gold particles (Con A-gold); (4) the localization of chitin oligomers using wheat germ agglutinin and ovomucoid labeled with gold particles (WGA-gold). The mannan-rich cell wall layers were progressively lost as shown by altered PA-TCH-SP reactivity and a diffuse pattern of staining with Con A-gold and monAb-gold. The de novo appearance of conspicuous amounts of glycogen-like particles near the plasmalemma and in the cell wall was interpreted as evidence of a reparative process of the yeast cell wall. Chitin was seemingly unaltered and readily demonstrated by the WGA-gold in the wall remnants of ghost cells.lld:pubmed
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pubmed-article:1940612pubmed:pagination587-99lld:pubmed
pubmed-article:1940612pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1940612pubmed:articleTitleHistochemical and immunochemical study of the fate of Candida albicans inside human neutrophil phagolysosomes.lld:pubmed
pubmed-article:1940612pubmed:affiliationDepartment of Microbiology and Immunology, Université de Montréal, Quebec, Canada.lld:pubmed
pubmed-article:1940612pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1940612pubmed:publicationTypeIn Vitrolld:pubmed
pubmed-article:1940612pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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