| pubmed-article:1939250 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1939250 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:1939250 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:1939250 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1939250 | lifeskim:mentions | umls-concept:C0001471 | lld:lifeskim |
| pubmed-article:1939250 | lifeskim:mentions | umls-concept:C0018321 | lld:lifeskim |
| pubmed-article:1939250 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:1939250 | pubmed:issue | 33 | lld:pubmed |
| pubmed-article:1939250 | pubmed:dateCreated | 1991-12-26 | lld:pubmed |
| pubmed-article:1939250 | pubmed:abstractText | A1 adenosine receptors and associated guanine nucleotide-binding proteins (G proteins) were purified from bovine cerebral cortex by affinity chromatography (Munshi, R., and Linden, J. (1989) J. Biol. Chem. 264, 14853-14859). In this study we have identified the pertussis toxin-sensitive G protein subunits that co-purify with A1 adenosine receptors by immunoblotting with specific antipeptide antisera. Gi alpha 1, Gi alpha 2, Go alpha, G beta 35, and G beta 36 were detected. Of the total [35S]guanosine 5'-O-(3-thio)triphosphate [( 35S]GTP gamma S) binding sites, Gi alpha 1 and Go alpha each accounted for greater than 37% whereas Gi alpha 2 comprised less than 13%. G beta 35 was found in excess over G beta 36. Low molecular mass (21-25 kDa) GTP-binding proteins were not detected. We also examined the characteristics of purified receptors and various purified bovine brain G proteins reconstituted into phospholipid vesicles. All three alpha-subunits restored GTP gamma S-sensitive high affinity binding of the agonist 125I-aminobenzyladenosine to a fraction (25%) of reconstituted receptors with a selectivity order of Gi2 greater than Go greater than or equal to Gi1 (ED50 values of G proteins measured as fold excess over the receptor concentration were 4.7 +/- 1.2, 24 +/- 5, and 34 +/- 7, respectively). Furthermore, receptors occupied with the agonist R-phenylisopropyladenosine catalytically increased the rate of binding of [35S]GTP gamma S to reconstituted G proteins by 6.5-8.5-fold. These results suggest that A1 adenosine receptors couple indiscriminately to pertussis toxin-sensitive G proteins. | lld:pubmed |
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| pubmed-article:1939250 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1939250 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1939250 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1939250 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1939250 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1939250 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1939250 | pubmed:author | pubmed-author:PangI HIH | lld:pubmed |
| pubmed-article:1939250 | pubmed:author | pubmed-author:LindenJJ | lld:pubmed |
| pubmed-article:1939250 | pubmed:author | pubmed-author:SternweisP... | lld:pubmed |
| pubmed-article:1939250 | pubmed:author | pubmed-author:MunshiRR | lld:pubmed |
| pubmed-article:1939250 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1939250 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1939250 | pubmed:volume | 266 | lld:pubmed |
| pubmed-article:1939250 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1939250 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1939250 | pubmed:pagination | 22285-9 | lld:pubmed |
| pubmed-article:1939250 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:1939250 | pubmed:meshHeading | pubmed-meshheading:1939250-... | lld:pubmed |
| pubmed-article:1939250 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1939250 | pubmed:articleTitle | A1 adenosine receptors of bovine brain couple to guanine nucleotide-binding proteins Gi1, Gi2, and Go. | lld:pubmed |
| pubmed-article:1939250 | pubmed:affiliation | Department of Internal Medicine (Cardiology), University of Virginia, Charlottesville 22908. | lld:pubmed |
| pubmed-article:1939250 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1939250 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1939250 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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