| pubmed-article:19380510 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C1280551 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C0019911 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C0475463 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C0002826 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C1265425 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C1705920 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C0439064 | lld:lifeskim |
| pubmed-article:19380510 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:19380510 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:19380510 | pubmed:dateCreated | 2009-9-1 | lld:pubmed |
| pubmed-article:19380510 | pubmed:abstractText | Hookworms digest hemoglobin from erythrocytes via a proteolytic cascade that begins with the aspartic protease, APR-1. Ac-APR-1 from the dog hookworm, Ancylostoma caninum, protects dogs against hookworm infection via antibodies that neutralize enzymatic activity and interrupt blood-feeding. Toward developing a human hookworm vaccine, we expressed both wild-type (Na-APR-1(wt)) and mutant (Na-APR-1(mut)-mutagenesis of the catalytic aspartic acids) forms of Na-APR-1 from the human hookworm, Necator americanus. Refolded Na-APR-1(wt) was catalytically active, and Na-APR-1(mut) was catalytically inactive but still bound substrates. Vaccination of canines with Na-APR-1(mut) and heterologous challenge with A. caninum resulted in significantly reduced parasite egg burdens (P=0.034) and weight loss (P=0.022). Vaccinated dogs also had less gut pathology, fewer adult worms, and reduced blood loss compared to controls but these did not reach statistical significance. Vaccination with Na-APR-1(mut) induced antibodies that bound the native enzyme in the parasite gut and neutralized enzymatic activity of Na-APR-1(wt) and APR-1 orthologues from three other hookworm species that infect humans. IgG1 against Na-APR-1(mut) was the most prominently detected antibody in sera from people resident in high-transmission areas for N. americanus, indicating that natural boosting may occur in exposed humans. Na-APR-1(mut) is now a lead antigen for the development of an antihematophagy vaccine for human hookworm disease. | lld:pubmed |
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| pubmed-article:19380510 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19380510 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19380510 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19380510 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19380510 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19380510 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:19380510 | pubmed:issn | 1530-6860 | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:HotezPeter... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:LoukasAlexA | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:ZhanBinB | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:JonesKathrynK | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:MilesAaron... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:BethonyJeffre... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:BottazziMaria... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:MulvennaJason... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:SmithTraceyT | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:PlieskattJord... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:PearsonMark... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:RanjitNajjuN | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:JiangDeshengD | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:KeeganBrianB | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:SantiagoHelto... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:TriboletLeonL | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:DermodyJessic... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:PickeringDarr... | lld:pubmed |
| pubmed-article:19380510 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:19380510 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19380510 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:19380510 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19380510 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19380510 | pubmed:pagination | 3007-19 | lld:pubmed |
| pubmed-article:19380510 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:meshHeading | pubmed-meshheading:19380510... | lld:pubmed |
| pubmed-article:19380510 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19380510 | pubmed:articleTitle | An enzymatically inactivated hemoglobinase from Necator americanus induces neutralizing antibodies against multiple hookworm species and protects dogs against heterologous hookworm infection. | lld:pubmed |
