| pubmed-article:19349972 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C0013570 | lld:lifeskim |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C0023621 | lld:lifeskim |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C0079941 | lld:lifeskim |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:19349972 | lifeskim:mentions | umls-concept:C0205198 | lld:lifeskim |
| pubmed-article:19349972 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19349972 | pubmed:dateCreated | 2009-4-8 | lld:pubmed |
| pubmed-article:19349972 | pubmed:abstractText | We present a yeast chemical-genomics approach designed to identify genes that when mutated confer drug resistance, thereby providing insight about the modes of action of compounds. We developed a molecular barcoded yeast open reading frame (MoBY-ORF) library in which each gene, controlled by its native promoter and terminator, is cloned into a centromere-based vector along with two unique oligonucleotide barcodes. The MoBY-ORF resource has numerous genetic and chemical-genetic applications, but here we focus on cloning wild-type versions of mutant drug-resistance genes using a complementation strategy and on simultaneously assaying the fitness of all transformants with barcode microarrays. The complementation cloning was validated by mutation detection using whole-genome yeast tiling microarrays, which identified unique polymorphisms associated with a drug-resistant mutant. We used the MoBY-ORF library to identify the genetic basis of several drug-resistant mutants and in this analysis discovered a new class of sterol-binding compounds. | lld:pubmed |
| pubmed-article:19349972 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19349972 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19349972 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19349972 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19349972 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19349972 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19349972 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19349972 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19349972 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19349972 | pubmed:issn | 1546-1696 | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:YoshidaMinoru... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:BooneCharlesC | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:BotsteinDavid... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:KohJudice L... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:AndersenRaymo... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:MagtanongLesl... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:NishimuraShin... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:GiaeverGuriG | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:AndrewsBrenda... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:GreshamDavidD | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:GrahamTodd... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:NislowCoreyC | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:GrayChristoph... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:NatarajanPara... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:PorterJustinJ | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:BarkerSarah... | lld:pubmed |
| pubmed-article:19349972 | pubmed:author | pubmed-author:HoCheuk HeiCH | lld:pubmed |
| pubmed-article:19349972 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19349972 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:19349972 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19349972 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19349972 | pubmed:pagination | 369-77 | lld:pubmed |
| pubmed-article:19349972 | pubmed:meshHeading | pubmed-meshheading:19349972... | lld:pubmed |
| pubmed-article:19349972 | pubmed:meshHeading | pubmed-meshheading:19349972... | lld:pubmed |
| pubmed-article:19349972 | pubmed:meshHeading | pubmed-meshheading:19349972... | lld:pubmed |
| pubmed-article:19349972 | pubmed:meshHeading | pubmed-meshheading:19349972... | lld:pubmed |
| pubmed-article:19349972 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19349972 | pubmed:articleTitle | A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. | lld:pubmed |
| pubmed-article:19349972 | pubmed:affiliation | Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. | lld:pubmed |
| pubmed-article:19349972 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19349972 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19349972 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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