| pubmed-article:19346473 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19346473 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:19346473 | lifeskim:mentions | umls-concept:C0017302 | lld:lifeskim |
| pubmed-article:19346473 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:19346473 | lifeskim:mentions | umls-concept:C0089069 | lld:lifeskim |
| pubmed-article:19346473 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:19346473 | pubmed:dateCreated | 2009-5-6 | lld:pubmed |
| pubmed-article:19346473 | pubmed:abstractText | We identified a fluorophore, 1-aminoanthracene (1-AMA), that is anesthetic, potentiates GABAergic transmission, and gives an appropriate dissociation constant, K(d) approximately 0.1 mM, for binding to the general anesthetic site in horse spleen apoferritin (HSAF). 1-AMA fluorescence is enhanced when bound to HSAF. Thus, displacement of 1-AMA from HSAF by other anesthetics attenuates the fluorescence signal and allows determination of K(d), as validated by isothermal titration calorimetry. This provides a unique fluorescence assay for compound screening and anesthetic discovery. Additional electrophysiology experiments in isolated cells indicate that 1-AMA potentiates chloride currents elicited by GABA, similar to many general anesthetics. Furthermore, 1-AMA reversibly immobilizes stage 45-50 Xenopus laevis tadpoles (EC(50) = 16 microM) and fluorescence micrographs show 1-AMA localized to brain and olfactory regions. Thus, 1-AMA provides an unprecedented opportunity for studying general anesthetic distribution in vivo at the cellular and subcellular levels. | lld:pubmed |
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| pubmed-article:19346473 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:19346473 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19346473 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19346473 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19346473 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:EckenhoffRode... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:MEESS | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:KleinMichael... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:Venkatachalan... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:DmochowskiIva... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:PearceRobert... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:BranniganGrac... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:ButtsChristop... | lld:pubmed |
| pubmed-article:19346473 | pubmed:author | pubmed-author:SaadAbdalla... | lld:pubmed |
| pubmed-article:19346473 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19346473 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:19346473 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:19346473 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19346473 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19346473 | pubmed:pagination | 6501-6 | lld:pubmed |
| pubmed-article:19346473 | pubmed:dateRevised | 2010-9-22 | lld:pubmed |
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| pubmed-article:19346473 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19346473 | pubmed:articleTitle | Identification of a fluorescent general anesthetic, 1-aminoanthracene. | lld:pubmed |
| pubmed-article:19346473 | pubmed:affiliation | Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA. | lld:pubmed |
