Linked Life Data

19342973

Source:http://linkedlifedata.com/resource/pubmed/id/19342973

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:19342973rdf:typepubmed:Citationlld:pubmed
pubmed-article:19342973lifeskim:mentionsumls-concept:C0009450lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0027651lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0011306lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0003320lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0042210lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C1280500lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0679622lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0449445lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C0205314lld:lifeskim
pubmed-article:19342973lifeskim:mentionsumls-concept:C1709100lld:lifeskim
pubmed-article:19342973pubmed:issue4lld:pubmed
pubmed-article:19342973pubmed:dateCreated2009-4-30lld:pubmed
pubmed-article:19342973pubmed:abstractTextTo develop an efficient dendritic cell (DC)-based immunotherapy protocol, we examined whether simultaneous pulsing of DCs with a given antigen and a third-party antigen could enhance their antigen presentation capacity. Purified splenic DCs of Balb/c mice were pulsed separately with immunoglobulin G, ovalbumin, conalbumin, P15 peptide of Mycobacterium tuberculosis, and prostate-specific antigen or double combinations of the aforementioned antigens. In some settings, DCs pulsed with 1 antigen were mixed equally with those pulsed with another antigen. Antigen-pulsed DCs were injected into the footpad of syngeneic mice and proliferation of whole, CD4 and CD8 depleted lymph node cells was measured after restimulation with cognate antigen. Antigen-specific production of interferon-gamma (IFNgamma) was tested in culture supernatants. Frequency of responding lymph node cells was determined by IFNgamma enzyme-linked immunosorbent spot assay. Our results showed that copulsing of DCs with 2 unrelated antigens increased the capacity of DCs to induce antigen-specific T-cell proliferation against both antigens up to 16-fold. Injection of 2 populations of DCs each pulsed with a different antigen, increased proliferation of primed T cells significantly as well. Both CD4 and CD8 depleted populations showed vigorous proliferative response in copulsing system. In addition, copulsing of DCs with 2 antigens resulted in higher frequency of antigen-specific responding cells and significantly more IFNgamma production. Our results clearly showed that unrelated peptides and proteins could be used to enhance efficacy of DC-based vaccines and in this system, each antigen served to help the other one, a condition that we termed as "mutual helper effect."lld:pubmed
pubmed-article:19342973pubmed:languageenglld:pubmed
pubmed-article:19342973pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19342973pubmed:citationSubsetIMlld:pubmed
pubmed-article:19342973pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19342973pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19342973pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19342973pubmed:statusMEDLINElld:pubmed
pubmed-article:19342973pubmed:monthMaylld:pubmed
pubmed-article:19342973pubmed:issn1537-4513lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:Jeddi-Tehrani...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:DokouhakiPoun...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:ZarnaniAmir...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:ShojaeianJale...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:AkhondiMohamm...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:GhodsRoyaRlld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:NikooShohrehSlld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:BozorgmehrMah...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:BayatAli...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:Ostadkarampou...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:MahmoudiAhmad...lld:pubmed
pubmed-article:19342973pubmed:authorpubmed-author:RezaniaSiminSlld:pubmed
pubmed-article:19342973pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19342973pubmed:volume32lld:pubmed
pubmed-article:19342973pubmed:ownerNLMlld:pubmed
pubmed-article:19342973pubmed:authorsCompleteYlld:pubmed
pubmed-article:19342973pubmed:pagination325-32lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:meshHeadingpubmed-meshheading:19342973...lld:pubmed
pubmed-article:19342973pubmed:year2009lld:pubmed
pubmed-article:19342973pubmed:articleTitleMutual helper effect in copulsing of dendritic cells with 2 antigens: a novel approach for improvement of dendritic-based vaccine efficacy against tumors and infectious diseases simultaneously.lld:pubmed
pubmed-article:19342973pubmed:affiliationDepartment of Reproductive Immunology, Reproductive Biotechnology Research Center, Avicenna Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.lld:pubmed
pubmed-article:19342973pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19342973pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed