| pubmed-article:19341422 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19341422 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:19341422 | lifeskim:mentions | umls-concept:C0036125 | lld:lifeskim |
| pubmed-article:19341422 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:19341422 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:19341422 | lifeskim:mentions | umls-concept:C1336789 | lld:lifeskim |
| pubmed-article:19341422 | lifeskim:mentions | umls-concept:C0205132 | lld:lifeskim |
| pubmed-article:19341422 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19341422 | pubmed:dateCreated | 2009-8-28 | lld:pubmed |
| pubmed-article:19341422 | pubmed:abstractText | Salmonella enterica serovar Typhi z66-positive strain contains an fljBA-like operon on a linear plasmid and the fliC gene on the chromosome encoding d or j antigen. The fljB-like gene has been identified as the gene encoding z66 antigen. To investigate the function of the fljA-like gene in S. enterica serovar Typhi, a z66-positive wild-type strain GIFU10007 and its phase variation j positive strain 007j+ were used in the present study. After deletion of the fljA-like gene, the wild-type strain that only expressed FljB:z66 could express both FliC:j and FljB:z66 at the same time. A recombinant plasmid, pBADfljA, containing the fljA-like gene was prepared. SDS-PAGE and western blot analysis of secreted proteins showed that when the strain 007j+ was transformed by the recombinant plasmid, pBADfljA, the expression of FliC:j was inhibited. The fliC::lacZ fusion of the strain 007j+ was constructed. Beta-Galactosidase activity was decreased approximately 100-fold after harboring the plasmid pBADfljA in the fliC::lacZ mutant strain. However, the fliC mRNA level investigated by RT-PCR was decreased only approximately seven-fold in the strain 007j+ after harboring the plasmid pBADfljA. These results demonstrated that thefljA-like gene on a linear plasmid of S. enterica serovar Typhi is a fljA gene, the repressor of fliC gene, and the inhibition by FljA is likely at the post-transcriptional level. | lld:pubmed |
| pubmed-article:19341422 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19341422 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19341422 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19341422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19341422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19341422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19341422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19341422 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19341422 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19341422 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19341422 | pubmed:issn | 0385-5600 | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:FryK EKE | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:XuHuaxiH | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:EzakiTakayuki... | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:ZhouLipingL | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:ShengXiumeiX | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:HuangXinxiang... | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:ZhangHaifangH | lld:pubmed |
| pubmed-article:19341422 | pubmed:author | pubmed-author:XuShungaoS | lld:pubmed |
| pubmed-article:19341422 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19341422 | pubmed:volume | 53 | lld:pubmed |
| pubmed-article:19341422 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19341422 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19341422 | pubmed:pagination | 191-7 | lld:pubmed |
| pubmed-article:19341422 | pubmed:meshHeading | pubmed-meshheading:19341422... | lld:pubmed |
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| pubmed-article:19341422 | pubmed:meshHeading | pubmed-meshheading:19341422... | lld:pubmed |
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| pubmed-article:19341422 | pubmed:meshHeading | pubmed-meshheading:19341422... | lld:pubmed |
| pubmed-article:19341422 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19341422 | pubmed:articleTitle | Identification of a fljA gene on a linear plasmid as the repressor gene of fliC in Salmonella enterica serovar Typhi. | lld:pubmed |
| pubmed-article:19341422 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, Jiangsu University School of Medical Technology, Zhenjiang, Jiangsu 212013, PR China. | lld:pubmed |
| pubmed-article:19341422 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19341422 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19341422 | lld:entrezgene |
