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pubmed-article:19322814pubmed:dateCreated2009-5-11lld:pubmed
pubmed-article:19322814pubmed:abstractTextNMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3-hydroxybutyrate-co-3-hydoxyvalerate) copolymers, P(3HB-co-3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB-co-3HV) was defined by analysis of their high-resolution 1D (13)C NMR and 2D (1)H/(13)C HSQC and (1)H/(13)C HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB-co-3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy.lld:pubmed
pubmed-article:19322814pubmed:languageenglld:pubmed
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pubmed-article:19322814pubmed:statusMEDLINElld:pubmed
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pubmed-article:19322814pubmed:issn1097-458Xlld:pubmed
pubmed-article:19322814pubmed:authorpubmed-author:LemosP CPClld:pubmed
pubmed-article:19322814pubmed:authorpubmed-author:IvanovaGGlld:pubmed
pubmed-article:19322814pubmed:authorpubmed-author:RamosA MAMlld:pubmed
pubmed-article:19322814pubmed:authorpubmed-author:CabritaE JEJlld:pubmed
pubmed-article:19322814pubmed:authorpubmed-author:SerafimL SLSlld:pubmed
pubmed-article:19322814pubmed:authorpubmed-author:ReisM A MMAlld:pubmed
pubmed-article:19322814pubmed:copyrightInfo2009 John Wiley & Sons, Ltd.lld:pubmed
pubmed-article:19322814pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19322814pubmed:volume47lld:pubmed
pubmed-article:19322814pubmed:ownerNLMlld:pubmed
pubmed-article:19322814pubmed:authorsCompleteYlld:pubmed
pubmed-article:19322814pubmed:pagination497-504lld:pubmed
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pubmed-article:19322814pubmed:year2009lld:pubmed
pubmed-article:19322814pubmed:articleTitleInfluence of feeding strategies of mixed microbial cultures on the chemical composition and microstructure of copolyesters P(3HB-co-3HV) analyzed by NMR and statistical analysis.lld:pubmed
pubmed-article:19322814pubmed:affiliationREQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.lld:pubmed
pubmed-article:19322814pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19322814pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed