Linked Life Data

19298862

Source:http://linkedlifedata.com/resource/pubmed/id/19298862

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pubmed-article:19298862pubmed:abstractTextThe 60S ribosomal protein L22 (GenBank accession no. EF990190) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 447 bps was found to encode a putative 148 amino acids protein which shares 90% and 80% identity with RPL22 genes from Aedes aegypti and Anopheles gambiae respectively. Real-time quantitative PCR analysis demonstrated that the transcription level of RPL22 in deltamethrin-resistant strain was 2.57 folds higher than in deltamethrin-susceptible strain of Cx. pipiens pallens. Overexpression of RPL22 in C6/36 cells showed that the deltamethrin-resistance was decreased in C6/36-RPL22 cell compared to the control. The mRNA level of cytochrome P450 6A1 (CYP6A1, GenBank accession no. FJ423553) showed that CYP6A1 was down-regulated in the C6/36 transfected with RPL22 (C6/36-RPL22) cells, suggesting that CYP6A1 was repressed by RPL22. Our study provides the first evidence that RPL22 may play some role in the regulation of deltamethrin-resistance in Cx. pipiens pallens.lld:pubmed
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pubmed-article:19298862pubmed:articleTitleCloning and characterization of 60S ribosomal protein L22 (RPL22) from Culex pipiens pallens.lld:pubmed
pubmed-article:19298862pubmed:affiliationDepartment of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 210029, PR China.lld:pubmed
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