| pubmed-article:19286939 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19286939 | lifeskim:mentions | umls-concept:C0079281 | lld:lifeskim |
| pubmed-article:19286939 | lifeskim:mentions | umls-concept:C1882561 | lld:lifeskim |
| pubmed-article:19286939 | lifeskim:mentions | umls-concept:C0006685 | lld:lifeskim |
| pubmed-article:19286939 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:19286939 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:19286939 | pubmed:dateCreated | 2009-5-4 | lld:pubmed |
| pubmed-article:19286939 | pubmed:abstractText | The roles of intracellular calcium concentration ([Ca(2+)](i)) and Ca(2+) sensitization in lipopolysaccharide (LPS)-induced vascular smooth muscle (VSM) hyporesponsiveness are incompletely understood. To investigate these roles, contraction responses to endothelin-1 (ET-1) and 80 mM KCl; relaxation responses to nifedipine; the expression levels of mRNAs of ET-1 and its receptors (ET(A) or ET(B)); the expression levels of protein kinase C (PKC) and phosphorylation of Rho kinase (ROKalpha), CPI-17, and myosin phosphatase target subunit-1 (MYPT1); and changes in aortic VSM cell [Ca(2+)](i) were measured in LPS-treated aortic rings from male Wistar rats (250-300 g). LPS (10 mug/ml, 20 h) decreased contraction induced by ET-1 (0.3-100 nM) or 80 mM KCl. LPS-induced hypocontractility was not observed in the absence of external Ca(2+), but LPS-treated aorta remained hypocontractile on subsequent stepwise restoration of extracellular Ca(2+) (0.01-10 mM). Vascular relaxation to nifedipine; mRNA expression levels of ET-1, ET(A), or ET(B); protein expression levels of PKC; and phosphorylation levels of ROKalpha, CPI-17, and MYPT1 were not affected by LPS. In isolated aortic VSM cells, ET-1 caused a transient initial increase in [Ca(2+)](i), followed by a maintained tonic increase in [Ca(2+)](i), which was decreased by LPS pretreatment and was dependent on external Ca(2+). Subsequent restoration of extracellular Ca(2+) increased [Ca(2+)](i), but this increase was lower in the LPS-treated group. This difference in response to extracellular Ca(2+) addition was not affected by diltiazem, but was abolished by SKF-96365. Therefore, LPS induces hyporeactivity to ET-1 in rat aorta that depends on external Ca(2+) influx through non-voltage-operated Ca(2+) channels, but not on ET-1 receptor expression or Ca(2+) sensitization. | lld:pubmed |
| pubmed-article:19286939 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19286939 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19286939 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19286939 | pubmed:month | May | lld:pubmed |
| pubmed-article:19286939 | pubmed:issn | 0363-6135 | lld:pubmed |
| pubmed-article:19286939 | pubmed:author | pubmed-author:SmirnovSergey... | lld:pubmed |
| pubmed-article:19286939 | pubmed:author | pubmed-author:WatsonMalcolm... | lld:pubmed |
| pubmed-article:19286939 | pubmed:author | pubmed-author:El-AwadyMoham... | lld:pubmed |
| pubmed-article:19286939 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19286939 | pubmed:volume | 296 | lld:pubmed |
| pubmed-article:19286939 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19286939 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19286939 | pubmed:pagination | H1408-15 | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:meshHeading | pubmed-meshheading:19286939... | lld:pubmed |
| pubmed-article:19286939 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19286939 | pubmed:articleTitle | Voltage-independent calcium channels mediate lipopolysaccharide-induced hyporeactivity to endothelin-1 in the rat aorta. | lld:pubmed |
| pubmed-article:19286939 | pubmed:affiliation | Dept. of Pharmacy and Pharmacology, Univ. of Bath, Bath BA2 7AY, UK. | lld:pubmed |
| pubmed-article:19286939 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19286939 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:24323 | entrezgene:pubmed | pubmed-article:19286939 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19286939 | lld:entrezgene |
