| pubmed-article:19278727 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0019944 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0021745 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:19278727 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:19278727 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19278727 | pubmed:dateCreated | 2010-8-5 | lld:pubmed |
| pubmed-article:19278727 | pubmed:abstractText | Interferon gamma (IFN-gamma) is a pleiotropic cytokine that is recognized as an important modulator of the immune response. To date, there is no report that prokaryocyte-derived recombinant equine IFN-gamma has antiviral activity. In this report, the gene coding equine IFN-gamma (EIFN-gamma) mature protein was cloned into pET-28a (+) and the recombinant EIFN-gamma was expressed in Escherichia coli (E. coli). The antiviral activity of expressed recombinant EIFN-gamma was evaluated by using a recombinant Vesicular Stomatitis Virus expressing green fluorescence protein (rVSV-GFP) system in the equine fetal kidney-78 cell line (EFK-78). The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with EIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titer of antiviral activity was 1 x 10(3)AU/ml. These results demonstrated that the EIFN-gamma expressed in this study had good biological activity. Pure forms and sufficient quantities of biologically active IFN-gamma could facilitate the study of its activities in modulating immune responses both in vivo and in vitro. | lld:pubmed |
| pubmed-article:19278727 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19278727 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19278727 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19278727 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19278727 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19278727 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19278727 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19278727 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19278727 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:19278727 | pubmed:issn | 1878-1667 | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:ChuFF | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:DonischE WEW | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:YORKG EGE | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:WangQunQ | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:BuZhigaoZ | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:LiuGuangliang... | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:ZhangWeijunW | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:ChenWeiyeW | lld:pubmed |
| pubmed-article:19278727 | pubmed:author | pubmed-author:TongTiegangT | lld:pubmed |
| pubmed-article:19278727 | pubmed:copyrightInfo | (c) 2009. Published by Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:19278727 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19278727 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:19278727 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19278727 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19278727 | pubmed:pagination | 333-42 | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:meshHeading | pubmed-meshheading:19278727... | lld:pubmed |
| pubmed-article:19278727 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19278727 | pubmed:articleTitle | Expression of biologically active recombinant equine interferon-gamma in Escherichia coli. | lld:pubmed |
| pubmed-article:19278727 | pubmed:affiliation | Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. | lld:pubmed |
| pubmed-article:19278727 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19278727 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
