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pubmed-article:19275222rdf:typepubmed:Citationlld:pubmed
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pubmed-article:19275222pubmed:issue4lld:pubmed
pubmed-article:19275222pubmed:dateCreated2009-4-24lld:pubmed
pubmed-article:19275222pubmed:abstractTextTo determine the biosynthesis pathway of the atisine-type diterpenoid alkaloids spiramines A/B and C/D, feeding experiments in in vitro cultured plantlets and enzymatic transformations in cell-free extracts were performed in combination with LCMS and tandem MS analyses. L-[2-(13)C,(15)N]Serine was used in the feeding experiments and enzymatic transformations, and the diterpene spiraminol was identified as a biosynthetic precursor of spiramine alkaloids. The LCMS and tandem MS spectra of the extracts from these experiments indicated that L-[2-(13)C,(15)N]serine was incorporated into spiramines A/B and C/D. The labeled reaction products show that l-serine is the one possible nitrogen source involved in the biosynthesis of atisine-type DAs.lld:pubmed
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pubmed-article:19275222pubmed:issn1520-6025lld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:RauJ LJLJrlld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:HeHong-PingHPlld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:ShenYue-MaoYMlld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:HaoXiao-Jiang...lld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:ZengYingYlld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:ZhaoPei-JiPJlld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:FanLi-MingLMlld:pubmed
pubmed-article:19275222pubmed:authorpubmed-author:NieJing-LeiJLlld:pubmed
pubmed-article:19275222pubmed:issnTypeElectroniclld:pubmed
pubmed-article:19275222pubmed:volume72lld:pubmed
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pubmed-article:19275222pubmed:pagination645-9lld:pubmed
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pubmed-article:19275222pubmed:year2009lld:pubmed
pubmed-article:19275222pubmed:articleTitleApproach to the biosynthesis of atisine-type diterpenoid alkaloids.lld:pubmed
pubmed-article:19275222pubmed:affiliationState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, People's Republic of China.lld:pubmed
pubmed-article:19275222pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19275222pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed