| pubmed-article:19271216 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0085470 | lld:lifeskim |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0599840 | lld:lifeskim |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0029073 | lld:lifeskim |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:19271216 | lifeskim:mentions | umls-concept:C0047466 | lld:lifeskim |
| pubmed-article:19271216 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:19271216 | pubmed:dateCreated | 2009-5-22 | lld:pubmed |
| pubmed-article:19271216 | pubmed:abstractText | To produce extracellular chiral 3-hydroxyacyl acids (3HA) by fermentation, a novel pathway was constructed by expressing tesB gene encoding thioesterase II into Pseudomonas putida KTOY01, which was a polyhydroxyalkanoate (PHA) synthesis operon knockout mutant. 3HA mixtures of 0.35 g/l consisting of 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydroxydodecanoate (3HDD) were produced in shake-flask study using dodecanoate as a sole carbon source. Additional knockout of fadB and fadA genes encoding 3-ketoacyl-CoA thiolase and 3-hydroxyacyl-CoA dehydrogenase in P. putida KTOY01 led to the weakening of the beta-oxidation pathway. The fadBA and PHA synthesis operon knockout mutant P. putida KTOY07 expressing tesB gene produced 2.44 g/l 3HA, significantly more than that of the beta-oxidation intact mutant. The 3HA mixture contained 90 mol% 3HDD as a dominant component. A fed-batch fermentation process carried out in a 6-l automatic fermentor produced 7.27 g/l extracellular 3HA containing 96 mol% fraction of 3HDD after 28 h of growth. For the first time, it became possible to produce 3HDD-dominant 3HA monomers. | lld:pubmed |
| pubmed-article:19271216 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19271216 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19271216 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19271216 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19271216 | pubmed:issn | 1432-0614 | lld:pubmed |
| pubmed-article:19271216 | pubmed:author | pubmed-author:SeckGG | lld:pubmed |
| pubmed-article:19271216 | pubmed:author | pubmed-author:ChenGuo-Qiang... | lld:pubmed |
| pubmed-article:19271216 | pubmed:author | pubmed-author:WuQiongQ | lld:pubmed |
| pubmed-article:19271216 | pubmed:author | pubmed-author:OuyangShao-Pi... | lld:pubmed |
| pubmed-article:19271216 | pubmed:author | pubmed-author:ChungAhleumA | lld:pubmed |
| pubmed-article:19271216 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19271216 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:19271216 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19271216 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19271216 | pubmed:pagination | 513-9 | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
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| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:meshHeading | pubmed-meshheading:19271216... | lld:pubmed |
| pubmed-article:19271216 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19271216 | pubmed:articleTitle | Microbial production of 3-hydroxydodecanoic acid by pha operon and fadBA knockout mutant of Pseudomonas putida KT2442 harboring tesB gene. | lld:pubmed |
| pubmed-article:19271216 | pubmed:affiliation | Beijing Key Laboratory of Protein Therapeutics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China. | lld:pubmed |
| pubmed-article:19271216 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19271216 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19271216 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19271216 | lld:pubmed |
