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pubmed-article:19269809pubmed:abstractTextSimultaneous biodegradation of hexachlorocyclohexane (HCH) and organophosphates (OPs) by a recombinant Stenotrophomonas sp. was studied in the study. The broad-host-range plasmid pVGAB, harboring enhanced green fluorescent protein gene (egfp) and dehalogenase genes (linA and linB), was constructed and transformed into the OP-degrading strain Stenotrophomonas YC-1 to get the recombinant strain YC-H. Over-expression of dehalogenase (LinA and LinB) and enhanced green fluorescent protein (EGFP) was obtained in YC-H by determining their enzymatic activities and fluorescence intensity. YC-H was capable of rapidly and simultaneously degrading 10mg/l gamma-HCH and 100mg/l methyl parathion (MP) determined by GC-ECD analysis. A bioremediation assay with YC-H inoculated into fumigated and nonfumigated soil showed that both 10mg/kg gamma-HCH and 100mg/kg MP could be completely degraded within 32 days. The novel EGFP-marked bacterium could be potentially applied in the field-scale decontamination of HCH and OPs residues in the environment.lld:pubmed
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pubmed-article:19269809pubmed:year2009lld:pubmed
pubmed-article:19269809pubmed:articleTitleDevelopment of an autofluorescent organophosphates-degrading Stenotrophomonas sp. with dehalogenase activity for the biodegradation of hexachlorocyclohexane (HCH).lld:pubmed
pubmed-article:19269809pubmed:affiliationState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.lld:pubmed
pubmed-article:19269809pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19269809pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed