| pubmed-article:19250642 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19250642 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:19250642 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:19250642 | lifeskim:mentions | umls-concept:C0035331 | lld:lifeskim |
| pubmed-article:19250642 | lifeskim:mentions | umls-concept:C0282548 | lld:lifeskim |
| pubmed-article:19250642 | lifeskim:mentions | umls-concept:C0769628 | lld:lifeskim |
| pubmed-article:19250642 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19250642 | pubmed:dateCreated | 2009-3-9 | lld:pubmed |
| pubmed-article:19250642 | pubmed:abstractText | Retinal leukostasis, mediated by intracellular adhesion molecule-1 (ICAM-1) and vascular endothelial growth factor (VEGF), has been implicated in the pathogenesis of early diabetic retinopathy. Phosphomannopentaose sulfate (PI-88) is a highly sulfonated oligosaccharide which inhibits heparanase activity and competes with heparan sulfate binding to growth factors. In this study, we evaluated whether PI-88 could inhibit retinal leukostasis in strepotzotocin(STZ)-induced diabetic rat and elucidated the possible mechanisms. Diabetes was induced in Sprague-Dawley rats by intraperitoneal injection (i.p.) of STZ. Three months after induction, diabetic rats were administered PI-88 (25 mg/kg body weight) or vehicle solution daily via i.p. for 14 consecutive days. Leukostasis was analyzed on retinal flatmounts by concanavalin A and CD45 immunofluorescence staining. Retinal function was analyzed by electroretinography (ERG). ICAM-1 and VEGF levels in retinas were studied by Western blot and enzyme-linked immunosorbent assay (ELISA) respectively. The systemic administration of PI-88, but not vehicle, significantly decreased the number of adherent leukocytes in retinas by 52.24% (P<0.001) and led to significant preservation (about 50%, P<0.001) of scotopic ERG a- and b-wave amplitudes in treated diabetic rats as compared to those of diabetic control rats. These changes were associated with downregulation of ICAM-1 (45%, P<0.001) and VEGF (26.83+/-2.01 versus 40.8+/-3.24 pg/mg, P<0.01) in retinas of PI-88 treated diabetic rats as compared to those of diabetic control rats. PI-88 significantly inhibited retinal leukostasis and reversed retinal dysfunction by a mechanism that may include decreased ICAM-1 and VEGF expression in diabetic rats. Our data suggests that PI-88 is a promising agent for the treatment of diabetic retinopathy. | lld:pubmed |
| pubmed-article:19250642 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19250642 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19250642 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19250642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19250642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19250642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19250642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19250642 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19250642 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19250642 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:19250642 | pubmed:issn | 1090-2104 | lld:pubmed |
| pubmed-article:19250642 | pubmed:author | pubmed-author:HuJieJ | lld:pubmed |
| pubmed-article:19250642 | pubmed:author | pubmed-author:TangShiboS | lld:pubmed |
| pubmed-article:19250642 | pubmed:author | pubmed-author:LuoYanY | lld:pubmed |
| pubmed-article:19250642 | pubmed:author | pubmed-author:MaPingpingP | lld:pubmed |
| pubmed-article:19250642 | pubmed:author | pubmed-author:ZhuXiaoboX | lld:pubmed |
| pubmed-article:19250642 | pubmed:author | pubmed-author:MaHongjieH | lld:pubmed |
| pubmed-article:19250642 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19250642 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:19250642 | pubmed:volume | 380 | lld:pubmed |
| pubmed-article:19250642 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19250642 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19250642 | pubmed:pagination | 402-6 | lld:pubmed |
| pubmed-article:19250642 | pubmed:meshHeading | pubmed-meshheading:19250642... | lld:pubmed |
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| pubmed-article:19250642 | pubmed:meshHeading | pubmed-meshheading:19250642... | lld:pubmed |
| pubmed-article:19250642 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19250642 | pubmed:articleTitle | Phosphomannopentaose sulfate (PI-88) inhibits retinal leukostasis in diabetic rat. | lld:pubmed |
| pubmed-article:19250642 | pubmed:affiliation | State Key Laboratory of Ophthalmology, Zhongshan Opthalmic Center, Sun Yat-sen University, 54S, Xianlie Road, Guangzhou 510060, China. | lld:pubmed |
| pubmed-article:19250642 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19250642 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
