Linked Life Data

19235210

Source:http://linkedlifedata.com/resource/pubmed/id/19235210

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pubmed-article:19235210pubmed:abstractTextWe tested the in vitro feasibility of porous PCL (poly(epsilon-caprolactone)) as a scaffold for cartilage tissue engineering from mesenchymal stem cells (MSCs) and determined the effects of various surface treatments. Three porous PCL scaffold modifications were examined: (1) PCL/Pluronic F127, (2) PCL/collagen, and (3) PCL/Pluronic F127/collagen, in addition to (4) PCL-only. MSCs (5 x 10(5)) were seeded in PCL scaffolds of pore size 100-150 microm, and after 3 weeks of in vitro culture, MSC-scaffolds were investigated for gross appearance, DNA amount, glycosaminoglycan (GAG) content, chondrogenic gene expression, and histology. Grossly, the cell-scaffold complexes became harder, and were more easily manipulated with a forceps after 3 weeks of culture. The three surface-treated scaffolds had higher DNA contents than did the PCL-only scaffold, and the GAG contents in PCL/collagen and PCL/F127/collagen scaffolds were higher than those seen in the PCL-only scaffold. Real-time PCR showed that Sox-9 and COL2A1 mRNA levels were remarkably elevated in PCL/collagen and PCL/F127/collagen scaffolds versus the PCL-only scaffold. On the other hand, Col1A1 and Col10A1 mRNA levels were lower in the three modified PCL scaffolds than in the PCL-only scaffold. Histological findings generally concurred with GAG and RT-PCR findings, and demonstrated the affinity of PCL-based scaffolds for MSCs and the potentials of these scaffold to induce chondrogenic differentiation. Cells showed more differentiated appearance and more abundant extracellular matrix formation in PCL/collagen and PCL/collagen/F127 scaffolds. Our findings suggest that PCL-based porous scaffolds may be useful carriers for MSC transplantation in the cartilage tissue engineering field, and that collagen-based surface modifications further enhance the chondrogenic differentiation of MSCs.lld:pubmed
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pubmed-article:19235210pubmed:authorpubmed-author:KimHye-JoungH...lld:pubmed
pubmed-article:19235210pubmed:authorpubmed-author:LeeJin-HoJHlld:pubmed
pubmed-article:19235210pubmed:copyrightInfo(c) 2009 Wiley Periodicals, Inc.lld:pubmed
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pubmed-article:19235210pubmed:year2010lld:pubmed
pubmed-article:19235210pubmed:articleTitleChondrogenesis using mesenchymal stem cells and PCL scaffolds.lld:pubmed
pubmed-article:19235210pubmed:affiliationDepartment of Orthopaedics, Dongguk University International Hospital, 814 Siksa-dong, Goyang 410-773, Korea.lld:pubmed
pubmed-article:19235210pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19235210pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed