| pubmed-article:19214390 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19214390 | lifeskim:mentions | umls-concept:C0997362 | lld:lifeskim |
| pubmed-article:19214390 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:19214390 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:19214390 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:19214390 | lifeskim:mentions | umls-concept:C0143595 | lld:lifeskim |
| pubmed-article:19214390 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:19214390 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:19214390 | pubmed:dateCreated | 2009-5-19 | lld:pubmed |
| pubmed-article:19214390 | pubmed:abstractText | Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (approximately 1 g/l) of extracellular glycosylated rSAK (approximately 18 kDa) with negligible plasminogen activation activity. Addition of tunicamycin during the induction phase resulted in expression of non-glycosylated and highly active rSAK (approximately 15 kDa) from the same clone. Two simple steps of ion-exchange chromatography produced an homogenous rSAK of >95% purity which suitable for future structural and functional studies. | lld:pubmed |
| pubmed-article:19214390 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19214390 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19214390 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19214390 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19214390 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19214390 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19214390 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19214390 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19214390 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19214390 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19214390 | pubmed:issn | 1573-6776 | lld:pubmed |
| pubmed-article:19214390 | pubmed:author | pubmed-author:PadmanabhanSr... | lld:pubmed |
| pubmed-article:19214390 | pubmed:author | pubmed-author:KumarJitendra... | lld:pubmed |
| pubmed-article:19214390 | pubmed:author | pubmed-author:MandalGoutamG | lld:pubmed |
| pubmed-article:19214390 | pubmed:author | pubmed-author:SoorapaneniSu... | lld:pubmed |
| pubmed-article:19214390 | pubmed:author | pubmed-author:PrasadBhaskar... | lld:pubmed |
| pubmed-article:19214390 | pubmed:author | pubmed-author:Apte-Deshpnad... | lld:pubmed |
| pubmed-article:19214390 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19214390 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:19214390 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19214390 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19214390 | pubmed:pagination | 811-7 | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:meshHeading | pubmed-meshheading:19214390... | lld:pubmed |
| pubmed-article:19214390 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19214390 | pubmed:articleTitle | High-level expression of non-glycosylated and active staphylokinase from Pichia pastoris. | lld:pubmed |
| pubmed-article:19214390 | pubmed:affiliation | Biotechnology R & D, Lupin Limited, 46A/47A, Nande Village, Mulshi Taluka, Pune 411042, India. | lld:pubmed |
| pubmed-article:19214390 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19214390 | lld:pubmed |
