| pubmed-article:19206545 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19206545 | lifeskim:mentions | umls-concept:C0011209 | lld:lifeskim |
| pubmed-article:19206545 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:19206545 | lifeskim:mentions | umls-concept:C0302350 | lld:lifeskim |
| pubmed-article:19206545 | lifeskim:mentions | umls-concept:C1710360 | lld:lifeskim |
| pubmed-article:19206545 | lifeskim:mentions | umls-concept:C0392752 | lld:lifeskim |
| pubmed-article:19206545 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19206545 | pubmed:dateCreated | 2009-2-11 | lld:pubmed |
| pubmed-article:19206545 | pubmed:abstractText | Nanomaterials such as block copolymeric membranes provide a platform for both cellular interrogation and biological mimicry. Their biomimetic properties are based upon the innate possession of hydrophilic and hydrophobic units that enable their integration with a broad range of therapeutic materials. As such, they can be engineered for specific applications in nanomedicine, including controlled/localized drug delivery. Here we describe a method for the functionalization of the polymethyloxazoline-polydimethylsiloxane-polymethyloxazoline (PMOXA-PDMS-PMOXA) block copolymer with anti-inflammatory molecules to develop copolymer-therapeutic hybrids, effectively conferring biological functionality to a versatile synthetic nanomembrane matrix and creating a platform for an anti-inflammatory drug delivery system. Utilizing self-assembly and Langmuir-Blodgett deposition methods, we mixed copolymers with dexamethasone (Dex), an anti-inflammatory glucocorticoid receptor agonist. The successful mixing of the copolymer with the drug was confirmed by surface pressure isotherms and fluorescence microscopy. Furthermore, at 4 nm thick per layer, orders of magnitude thinner than conventional drug delivery coatings, these dexamethasone-copolymer mixtures (PolyDex) suppressed in vitro expression of the inflammatory cytokines/signaling elements interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor alpha (TNFalpha), inducible nitric oxide synthase (iNOS), and interferon gamma inducible protein (IP-10). Finally, PolyDex maintained its anti-inflammatory properties in vivo confirmed through punch biopsies with tissue imagery via hematoxylin/eosin and macrophage specific staining using CD11b. Thus, we demonstrated that PolyDex may be utilized as a localized, highly efficient drug-copolymer composite for active therapeutic delivery to confer anti-inflammatory protection or as a platform material for broad drug elution capabilities. | lld:pubmed |
| pubmed-article:19206545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19206545 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19206545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19206545 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19206545 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19206545 | pubmed:issn | 1936-086X | lld:pubmed |
| pubmed-article:19206545 | pubmed:author | pubmed-author:ChengGenhongG | lld:pubmed |
| pubmed-article:19206545 | pubmed:author | pubmed-author:PierstorffEri... | lld:pubmed |
| pubmed-article:19206545 | pubmed:author | pubmed-author:HoDeanD | lld:pubmed |
| pubmed-article:19206545 | pubmed:author | pubmed-author:ChowEdward... | lld:pubmed |
| pubmed-article:19206545 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19206545 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:19206545 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19206545 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19206545 | pubmed:pagination | 33-40 | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:meshHeading | pubmed-meshheading:19206545... | lld:pubmed |
| pubmed-article:19206545 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:19206545 | pubmed:articleTitle | Copolymeric nanofilm platform for controlled and localized therapeutic delivery. | lld:pubmed |
| pubmed-article:19206545 | pubmed:affiliation | Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90095, USA. | lld:pubmed |
| pubmed-article:19206545 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19206545 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19206545 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
