| pubmed-article:19181623 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C0239946 | lld:lifeskim |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C0005516 | lld:lifeskim |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C0036825 | lld:lifeskim |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C1512223 | lld:lifeskim |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:19181623 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:19181623 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:19181623 | pubmed:dateCreated | 2009-5-4 | lld:pubmed |
| pubmed-article:19181623 | pubmed:abstractText | Liver fibrosis is currently assessed by liver biopsy, a costly and rather cumbersome procedure that is unsuitable for frequent patient monitoring, which drives research into biomarkers for this purpose. To investigate whether the serum N-glycome contains information suitable for this goal, we developed a 96-well plate-based serum N-glycomics sample preparation protocol that only involves fluid transfer steps and incubations in a PCR thermocycler yielding 8-aminopyrene-1,3,6-trisulfonic acid-labeled N-glycans. These N-glycans are then ready for analysis on the capillary electrophoresis-based DNA sequencers that are the current standard in clinical genetics laboratories worldwide. Subsequently we performed a multicenter, blinded study of 376 consecutive chronic hepatitis C virus patients for which liver biopsies and extensive serum biochemistry data were available. Among patients, the METAVIR fibrosis stage distribution was as follows: 10.6% F0, 44.4% F1, 20.5% F2, 18.4% F3, and 6.1% F4. We found that the ratio of two N-glycans, here called GlycoFibroTest, correlates with the histological fibrosis stage equally well as FibroTest (rho = 0.4-0.5 in F1-F4), which is used in the clinic today. Finally using affinity chromatography we depleted sera of immunoglobulin G, and this resulted in a complete removal of the undergalactosylated biantennary glycans from the N-glycome, which are partially determining GlycoFibroTest. | lld:pubmed |
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| pubmed-article:19181623 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:19181623 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19181623 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19181623 | pubmed:month | May | lld:pubmed |
| pubmed-article:19181623 | pubmed:issn | 1535-9484 | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:HalfonPhilipp... | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:LaroyWouterW | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:CallewaertNic... | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:DelangheJoris... | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:SablonErwinE | lld:pubmed |
| pubmed-article:19181623 | pubmed:author | pubmed-author:Vanderschaegh... | lld:pubmed |
| pubmed-article:19181623 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19181623 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:19181623 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19181623 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19181623 | pubmed:pagination | 986-94 | lld:pubmed |
| pubmed-article:19181623 | pubmed:dateRevised | 2010-9-23 | lld:pubmed |
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| pubmed-article:19181623 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19181623 | pubmed:articleTitle | GlycoFibroTest is a highly performant liver fibrosis biomarker derived from DNA sequencer-based serum protein glycomics. | lld:pubmed |
| pubmed-article:19181623 | pubmed:affiliation | Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, Flanders Institute for Biotechnology (VIB), Technologiepark 927, B-9052 Ghent, Belgium. | lld:pubmed |
| pubmed-article:19181623 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19181623 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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