| pubmed-article:1917930 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0009368 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0332157 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0031669 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0439590 | lld:lifeskim |
| pubmed-article:1917930 | lifeskim:mentions | umls-concept:C0070750 | lld:lifeskim |
| pubmed-article:1917930 | pubmed:issue | 27 | lld:pubmed |
| pubmed-article:1917930 | pubmed:dateCreated | 1991-10-29 | lld:pubmed |
| pubmed-article:1917930 | pubmed:abstractText | The T84 colonic cell line, a cultured Cl- secretory cell, elevates intracellular free Ca2+ [( Ca2+]i) in a concentration-dependent manner when exposed to carbachol or histamine. As determined with a fluorescence microscope imaging system, exposure of T84 cells to 100 microM carbachol or histamine resulted in an immediate [Ca2+]i rise of approximately 50-80 nM in all cells. Preincubation of monolayers for 1 h or longer with 0.4 microM phorbol 12,13-dibutyrate (PDB) reduced the number of cells which responded to histamine or carbachol and reduced the magnitude of the increase in the responding cells. This effect reached its maximum after 2 h and persisted for at least 24 h of PDB incubation. Binding of quinuclidinyl benzilate, a cholinergic receptor antagonist, indicated that down-regulation of external receptors was not an explanation for this effect. Examination of phospholipase C activity in T84 cell membranes showed increased basal activity in PDB-treated compared with control cells. Measurement of inositol phosphates generated by intact cells using myo-[3H]inositol incorporation or receptor binding assays showed that 2 h of incubation with PDB elevated basal levels of inositol 1,4,5-trisphosphate and prevented any further carbachol-induced generation of inositol trisphosphate. Probably as a consequence, both total cell calcium and Ca2+ ionophore-releasable calcium were decreased after 2 h of PDB incubation. Membrane-associated protein kinase C activity was elevated after a 2 h exposure to PDB but was below the level of detection after 24 h with PDB. Protein kinase C antagonists neither duplicated nor blocked the uncoupling of carbachol receptors induced by long term treatment with PDB. The results suggest that prolonged PDB incubation caused uncoupling and elevation of phospholipase C activity from cholinergic and histaminergic receptor regulation resulting in increased basal levels of inositol 1,4,5-trisphosphate. Protein kinase C apparently is not involved directly in the mechanism that leads to these effects. | lld:pubmed |
| pubmed-article:1917930 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1917930 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917930 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1917930 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1917930 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:SharpG WGW | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:WatsonAA | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:DonowitzMM | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:ZahniserDD | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:BreenD HDH | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:ReinlibLL | lld:pubmed |
| pubmed-article:1917930 | pubmed:author | pubmed-author:HomaidanFF | lld:pubmed |
| pubmed-article:1917930 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1917930 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1917930 | pubmed:volume | 266 | lld:pubmed |
| pubmed-article:1917930 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1917930 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1917930 | pubmed:pagination | 17904-11 | lld:pubmed |
| pubmed-article:1917930 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:1917930 | pubmed:meshHeading | pubmed-meshheading:1917930-... | lld:pubmed |
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| pubmed-article:1917930 | pubmed:meshHeading | pubmed-meshheading:1917930-... | lld:pubmed |
| pubmed-article:1917930 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1917930 | pubmed:articleTitle | Uncoupling of phospholipase C from receptor regulation of [Ca2+]i in T84 colonic cells by prolonged exposure to phorbol dibutyrate. | lld:pubmed |
| pubmed-article:1917930 | pubmed:affiliation | Department of Medicine (Gastroenterology Division), Johns Hopkins University School of Medicine, Baltimore, Maryland 21205. | lld:pubmed |
| pubmed-article:1917930 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1917930 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1917930 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1917930 | lld:pubmed |
