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pubmed-article:19169886pubmed:abstractTextPhosphatidylglycerol (PG) was synthesized from several phosphatidylcholines (PCs) via phospholipase D (PLD)-catalyzed transphosphatidylation in an aqueous system. The yield of PG were 71 and 68 mol% from soybean PC and egg yolk PC, respectively, under the optimum reaction conditions of 50 micromol PC, 10 mmol glycerol, 3 ml of acetate buffer, 1.6 U PLD, and 30 micromol CaCl(2) at 37 degrees C for 48 h. In case of salmon roe PC with 14.3% eicosapentaenoic acid and 26.8% docosahexaenoic acid, the PG yield increased to 94 mol% by addition of 46 mumol alpha-tocopherol, although the PG yield was only 10% in absence of alpha-tocopherol.lld:pubmed
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pubmed-article:19169886pubmed:authorpubmed-author:HosokawaMasas...lld:pubmed
pubmed-article:19169886pubmed:authorpubmed-author:MiyashitaKazu...lld:pubmed
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pubmed-article:19169886pubmed:authorpubmed-author:SuzuriKounosu...lld:pubmed
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pubmed-article:19169886pubmed:year2009lld:pubmed
pubmed-article:19169886pubmed:articleTitleEffect of alpha-tocopherol on the synthesis of phosphatidylglycerol catalyzed by phospholipase D in an aqueous system.lld:pubmed
pubmed-article:19169886pubmed:affiliationFaculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato, Hakodate, Hokkaido, 041-8611, Japan.lld:pubmed
pubmed-article:19169886pubmed:publicationTypeJournal Articlelld:pubmed