| pubmed-article:19165858 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19165858 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:19165858 | lifeskim:mentions | umls-concept:C0140080 | lld:lifeskim |
| pubmed-article:19165858 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:19165858 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:19165858 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:19165858 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:19165858 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:19165858 | pubmed:dateCreated | 2009-3-30 | lld:pubmed |
| pubmed-article:19165858 | pubmed:abstractText | IGF-1 receptor (IGF-1R) plays a key role in the development of numerous tumors. Blockade of IGF-1R axis using monoclonal antibodies constitutes an interesting approach to inhibit tumor growth. We have previously shown that h7C10, a humanized anti-IGF-1R Mab, exhibited potent antitumor activity in vivo. However, mechanisms of action of h7C10 are still unknown. Here, we showed that h7C10 inhibited IGF-1-induced IGF-1R phosphorylation in a dose-dependent manner. Also, h7C10 abolished IGF-1-induced activation of PI3K/AKT and MAPK pathways. Cell cycle progression and colony formation were affected in the presence of h7C10 probably because of the inhibition of IGF-1-induced cyclin D1 and E expression. In addition, we demonstrated that h7C10 induced a rapid IGF-1R internalization leading to an accumulation into cytoplasm resulting in receptor degradation. Using lysosome and proteasome inhibitors, we observed that the IGF-1R alpha- and beta-chains could follow different degradation routes. Thus, we demonstrated that antitumoral properties of h7C10 are the result of IGF-1-induced cell signaling inhibition and down-regulation of IGF-1R level suggesting that h7C10 could be a candidate for therapeutic applications. | lld:pubmed |
| pubmed-article:19165858 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19165858 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19165858 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19165858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19165858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19165858 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19165858 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19165858 | pubmed:month | May | lld:pubmed |
| pubmed-article:19165858 | pubmed:issn | 1097-0215 | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:DupontJoëlleJ | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:CorvaïaNathal... | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:BlaeckeAlineA | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:BroussasMatth... | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:GoetschLilian... | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:GonzalezAlexa... | lld:pubmed |
| pubmed-article:19165858 | pubmed:author | pubmed-author:FournierMathi... | lld:pubmed |
| pubmed-article:19165858 | pubmed:copyrightInfo | (c) 2008 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:19165858 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19165858 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:19165858 | pubmed:volume | 124 | lld:pubmed |
| pubmed-article:19165858 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19165858 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19165858 | pubmed:pagination | 2281-93 | lld:pubmed |
| pubmed-article:19165858 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:19165858 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19165858 | pubmed:articleTitle | Molecular mechanisms involved in activity of h7C10, a humanized monoclonal antibody, to IGF-1 receptor. | lld:pubmed |
| pubmed-article:19165858 | pubmed:affiliation | Centre d'Immunologie Pierre Fabre, 5 Avenue Napoléon III, BP 60497, Saint-Julien-en-Genevois, France. matthieu.broussas@pierre-fabre.com | lld:pubmed |
| pubmed-article:19165858 | pubmed:publicationType | Journal Article | lld:pubmed |
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