| pubmed-article:19147115 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C0035567 | lld:lifeskim |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C0036563 | lld:lifeskim |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C0003069 | lld:lifeskim |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C1704222 | lld:lifeskim |
| pubmed-article:19147115 | lifeskim:mentions | umls-concept:C1517294 | lld:lifeskim |
| pubmed-article:19147115 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19147115 | pubmed:dateCreated | 2009-1-16 | lld:pubmed |
| pubmed-article:19147115 | pubmed:abstractText | Transgenic rice plants that accumulate the hypocholesterolemic pentapeptide lactostatin (IIAEK) as a fusion protein with the endosperm seed storage protein glutelin were developed. Plants were selected from Agrobacterium-mediated transformants using a mutated-rice acetolactate synthase (mALS) marker protein expressed under the control of the rice callus-specific promoter (CSP) (CSP:mALS). Lactostatin accumulation levels were compared in mature seeds of 78 independent transgenic rice lines selected for either the CSP:mALS gene cassette or hygromycin phosphotransferase (HPT) under the control of the CaMV 35S promoter (35S:HPT). Transgenic rice seeds harboring a CSP:mALS gene cassette accumulated more (approximately 120-200% on average) lactostatin than those with a 35S:HPT gene cassette, and accumulation was largely independent of transgene copy number. Furthermore, transgenic rice seeds that were selected by HPT under the control of CSP (CSP:HPT) also accumulated more lactostatin (approximately 160% on average) than transgenic rice seeds with the 35S:HPT gene cassette. These results indicate that high-value bioactive peptides such as lactostatin can be produced at higher levels using callus-specific selection than by conventional constitutive selection. Specific expression of a marker gene at the selection stage may thus result in increased target peptide accumulation levels in seeds. | lld:pubmed |
| pubmed-article:19147115 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19147115 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19147115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19147115 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19147115 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19147115 | pubmed:issn | 1347-4421 | lld:pubmed |
| pubmed-article:19147115 | pubmed:author | pubmed-author:TakaiwaFumioF | lld:pubmed |
| pubmed-article:19147115 | pubmed:author | pubmed-author:OzawaKenjirou... | lld:pubmed |
| pubmed-article:19147115 | pubmed:author | pubmed-author:WakasaYuhyaY | lld:pubmed |
| pubmed-article:19147115 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19147115 | pubmed:volume | 107 | lld:pubmed |
| pubmed-article:19147115 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19147115 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19147115 | pubmed:pagination | 78-83 | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:meshHeading | pubmed-meshheading:19147115... | lld:pubmed |
| pubmed-article:19147115 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19147115 | pubmed:articleTitle | Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system. | lld:pubmed |
| pubmed-article:19147115 | pubmed:affiliation | Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 3-1-3, Tsukuba, Ibaraki 305-8604, Japan. | lld:pubmed |
| pubmed-article:19147115 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19147115 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
