| pubmed-article:19144911 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19144911 | lifeskim:mentions | umls-concept:C1099354 | lld:lifeskim |
| pubmed-article:19144911 | lifeskim:mentions | umls-concept:C1705920 | lld:lifeskim |
| pubmed-article:19144911 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:19144911 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:19144911 | lifeskim:mentions | umls-concept:C1519595 | lld:lifeskim |
| pubmed-article:19144911 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19144911 | pubmed:dateCreated | 2009-1-15 | lld:pubmed |
| pubmed-article:19144911 | pubmed:abstractText | siRNAs mediate sequence-specific gene silencing in cultured mammalian cells but also silence unintended transcripts. Many siRNA off-target transcripts match the guide-strand "seed region," similar to the way microRNAs match their target sites. The extent to which this seed-matched, microRNA-like, off-target silencing affects the specificity of therapeutic siRNAs in vivo is currently unknown. Here, we compare microRNA-like off-target regulations in mouse liver in vivo with those seen in cell culture for a series of therapeutic candidate siRNAs targeting Apolipoprotein B (APOB). Each siRNA triggered regulation of consistent microRNA-like off-target transcripts in mouse livers and in cultured mouse liver tumor cells. In contrast, there was only random overlap between microRNA-like off-target transcripts from cultured human and mouse liver tumor cells. Therefore, siRNA therapeutics may trigger microRNA-like silencing of many unintended targets in vivo, and the potential toxicities caused by these off-target gene regulations cannot be accurately assessed in rodent models. | lld:pubmed |
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| pubmed-article:19144911 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19144911 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19144911 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19144911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19144911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19144911 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19144911 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19144911 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19144911 | pubmed:issn | 1469-9001 | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:DaiHongyueH | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:LinsleyPeter... | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:BartzSteven... | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:SachsAlan BAB | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:BurchardJulja... | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:NeedhamRachel... | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:JacksonAimee... | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:TanYejunY | lld:pubmed |
| pubmed-article:19144911 | pubmed:author | pubmed-author:MalkovVladisl... | lld:pubmed |
| pubmed-article:19144911 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19144911 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:19144911 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19144911 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19144911 | pubmed:pagination | 308-15 | lld:pubmed |
| pubmed-article:19144911 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:19144911 | pubmed:meshHeading | pubmed-meshheading:19144911... | lld:pubmed |
| pubmed-article:19144911 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19144911 | pubmed:articleTitle | MicroRNA-like off-target transcript regulation by siRNAs is species specific. | lld:pubmed |
| pubmed-article:19144911 | pubmed:affiliation | Rosetta Inpharmatics LLC, Seattle, Washington 98109, USA. | lld:pubmed |
| pubmed-article:19144911 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19144911 | pubmed:publicationType | Comparative Study | lld:pubmed |
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