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pubmed-article:19137071pubmed:abstractTextIntact adult photoreceptors in culture can be a valuable tool in the search of therapies for retinal degenerations. The major challenge in this technique is that photoreceptors undergo an alteration in cytoarchitecture and loss of outer segment during the cell culture process. This study compared techniques for the isolation of photoreceptor cells from adult rat retinas to determine which technique yields the highest percent of structurally well preserved cells in vitro. In addition, the role of light exposure during the dissociation and culture process was investigated to minimize photoreceptor cell deformation over time in culture.lld:pubmed
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pubmed-article:19137071pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:19137071pubmed:articleTitlePreservation of intact adult rat photoreceptors in vitro: study of dissociation techniques and the effect of light.lld:pubmed
pubmed-article:19137071pubmed:affiliationDepartment of Biomedical Engineering, Pritzker Institute of Biomedical Science and Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.lld:pubmed
pubmed-article:19137071pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19137071pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed