| pubmed-article:19136555 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C1171311 | lld:lifeskim |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C0600448 | lld:lifeskim |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C1534709 | lld:lifeskim |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:19136555 | lifeskim:mentions | umls-concept:C0597551 | lld:lifeskim |
| pubmed-article:19136555 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:19136555 | pubmed:dateCreated | 2009-3-2 | lld:pubmed |
| pubmed-article:19136555 | pubmed:abstractText | Conventional split inteins have been useful for trans-splicing between recombinant proteins, and an artificial S1 split intein is useful for adding synthetic peptide onto the N terminus of recombinant proteins. Here we have engineered a novel S11 split intein for trans-splicing synthetic peptide onto the C terminus of recombinant proteins. The C-intein of the S11 split intein is extremely small (6 amino acids (aa)); thus it can easily be produced together with a synthetic C-extein to be added to the C terminus of target proteins. The S11 intein was derived from the Ssp GyrB intein after deleting the homing endonuclease domain and splitting the remaining intein sequence near the C terminus, producing a 150-aa N-intein (IN) and a 6-aa C-intein (IC). Its trans-splicing activity was demonstrated first in Escherichia coli cells and then in vitro for trans-splicing between a synthetic peptide and a recombinant protein. The in vitro trans-splicing reaction exhibited a typical rate constant of (6.9+/-2.2)x10(-5) s(-1) and reached a high efficiency of approximately 80%. This S11 split intein can be useful for adding any desirable chemical groups to the C terminus of a protein of interest, which may include modified and unnatural amino acids, biotin and fluorescent labels, and even drug molecules. | lld:pubmed |
| pubmed-article:19136555 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19136555 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19136555 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19136555 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19136555 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19136555 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19136555 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19136555 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:19136555 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:19136555 | pubmed:author | pubmed-author:LiuXiang-QinX... | lld:pubmed |
| pubmed-article:19136555 | pubmed:author | pubmed-author:ZhouKaisongK | lld:pubmed |
| pubmed-article:19136555 | pubmed:author | pubmed-author:ApplebyJulia... | lld:pubmed |
| pubmed-article:19136555 | pubmed:author | pubmed-author:VolkmannGerri... | lld:pubmed |
| pubmed-article:19136555 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19136555 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:19136555 | pubmed:volume | 284 | lld:pubmed |
| pubmed-article:19136555 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19136555 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19136555 | pubmed:pagination | 6194-9 | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:meshHeading | pubmed-meshheading:19136555... | lld:pubmed |
| pubmed-article:19136555 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19136555 | pubmed:articleTitle | Novel split intein for trans-splicing synthetic peptide onto C terminus of protein. | lld:pubmed |
| pubmed-article:19136555 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada. | lld:pubmed |
| pubmed-article:19136555 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19136555 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19136555 | lld:pubmed |
