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pubmed-article:19086295pubmed:abstractTextG-proteins (guanine nucleotide-binding proteins that usually exhibit GTPase activities) and related signal transduction processes play important roles in mediating plant defense responses; here, a rice (Oryza sativa) cDNA clone, OsGAP1, encoding a GTPase-activating protein (GAP) that also contains a protein kinase C conserved region 2 (C2) domain is reported. An interacting G-protein partner for the OsGAP1 protein was identified by yeast two-hybrid library screening and confirmed by co-immunoprecipitation; the GTPase-activation activity of OsGAP1 on this interacting G-protein was demonstrated using in vitro assays. OsGAP1 was induced by wounding in rice and the presence of the R locus Xa14 enhances such induction. Gain-of-function tests in transgenic rice and Arabidopsis thaliana showed that constitutive expression of OsGAP1 led to increased resistance to bacterial pathogens in both monocots and dicots.lld:pubmed
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pubmed-article:19086295pubmed:authorpubmed-author:HSUJ ZJZlld:pubmed
pubmed-article:19086295pubmed:authorpubmed-author:ZhangQiQlld:pubmed
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pubmed-article:19086295pubmed:authorpubmed-author:SunSamuel...lld:pubmed
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pubmed-article:19086295pubmed:articleTitleConstitutive expression of a rice GTPase-activating protein induces defense responses.lld:pubmed
pubmed-article:19086295pubmed:affiliationDepartment of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region.lld:pubmed
pubmed-article:19086295pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19086295pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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