| pubmed-article:19084662 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19084662 | lifeskim:mentions | umls-concept:C0014912 | lld:lifeskim |
| pubmed-article:19084662 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19084662 | lifeskim:mentions | umls-concept:C0018026 | lld:lifeskim |
| pubmed-article:19084662 | lifeskim:mentions | umls-concept:C1450054 | lld:lifeskim |
| pubmed-article:19084662 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19084662 | pubmed:dateCreated | 2008-12-16 | lld:pubmed |
| pubmed-article:19084662 | pubmed:abstractText | Low picograms of the hormone 17beta-estradiol were detected at an electrochemical immunosensor. This immunosensor features a gold nanoparticle|Protein G-(LC-SPDP)(1)-scaffold, to which a monoclonal anti-estradiol capture antibody was immobilised to facilitate a competitive immunoassay between sample 17beta-estradiol and a horseradish peroxidase-labelled 17beta-estradiol conjugate. Upon constructing this molecular architecture on a disposable gold electrode in a flow cell, amperometry was conducted to monitor the reduction current of benzoquinone produced from a catalytic reaction of horseradish peroxidase. This current was then quantitatively related to 17beta-estradiol present in a sample. Calibration of immunosensors in blood serum samples spiked with 17beta-estradiol yielded a linear response up to approximately 1200 pg mL(-1), a sensitivity of 0.61microA/pg mL(-1) and a detection limit of 6 pg mL(-1). We attribute these favourable characteristics of the immunosensors to the gold nanoparticle|Protein G-(LC-SPDP) scaffold, where the gold nanoparticles provided a large electrochemically active surface area that permits immobilisation of an enhanced quantity of all components of the molecular architecture, while the Protein G-(LC-SPDP) component aided in not only reducing steric hindrance when Protein G binds to the capture antibody, but also providing an orientation-controlled immobilisation of the capture antibody. Coupled with amperometric detection in a flow system, the immunosensor exhibited excellent reproducibility. | lld:pubmed |
| pubmed-article:19084662 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19084662 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19084662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19084662 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19084662 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19084662 | pubmed:issn | 1873-3573 | lld:pubmed |
| pubmed-article:19084662 | pubmed:author | pubmed-author:LiuXiaoqiangX | lld:pubmed |
| pubmed-article:19084662 | pubmed:author | pubmed-author:WongDanny K... | lld:pubmed |
| pubmed-article:19084662 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19084662 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:19084662 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:19084662 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19084662 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19084662 | pubmed:pagination | 1437-43 | lld:pubmed |
| pubmed-article:19084662 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:meshHeading | pubmed-meshheading:19084662... | lld:pubmed |
| pubmed-article:19084662 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19084662 | pubmed:articleTitle | Picogram-detection of estradiol at an electrochemical immunosensor with a gold nanoparticle|Protein G-(LC-SPDP)-scaffold. | lld:pubmed |
| pubmed-article:19084662 | pubmed:affiliation | Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. | lld:pubmed |
| pubmed-article:19084662 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19084662 | lld:pubmed |
