| pubmed-article:19073334 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C0949858 | lld:lifeskim |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C0017366 | lld:lifeskim |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C0017260 | lld:lifeskim |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C0037017 | lld:lifeskim |
| pubmed-article:19073334 | lifeskim:mentions | umls-concept:C1518422 | lld:lifeskim |
| pubmed-article:19073334 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19073334 | pubmed:dateCreated | 2009-2-24 | lld:pubmed |
| pubmed-article:19073334 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19073334 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19073334 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19073334 | pubmed:abstractText | RT-PCR using a commercial kit for yellow head virus (YHV) detection in growth-retarded shrimp yielded an unusual 777 bp amplicon instead of expected amplicons of 277 bp for YHV type-1 (YHV-1) or 406 bp for YHV type-2 (YHV-2). Cloning and sequencing (GenBank EU170438) revealed approximately 80% identity to non-structural (NS) ORF1b sequences of both YHV-1 (GenBank AA083987) and YHV-2 (GenBank AF227196), indicating an atypical YHV type (A-YHV) phylogenetically equidistant from both types. An RT-PCR test specifically designed for A-YHV revealed that it was uncommon and that its occurrence in shrimp culture ponds did not correlate with growth retardation or mortality. By immunohistochemistry with YHV-specific monoclonal antibodies, the A-YHV gave positive reactions for envelope protein gp64 and capsid protein p20, but not for envelope protein gp116, even though gp116 and gp64 originate from a polyprotein of ORF3. Lack of gp116 immunoreactivity correlated with a large ORF3 deletion (GenBank EU123854) in the region of the protein targeted by an MAb against gp116. Transmission electron microscopy of A-YHV-infected shrimp revealed only unenveloped pre-virions. During manuscript revision, information received revealed that typing of YHV isolates based on sequences of ORF1b and ORF3 had yielded several geographical types, including one virulent type (YHV-1b) with an ORF3 deletion sequence that matched the sequence of A-YHV. Using these sequences and an additional A-YHV sequence (EU853170) from the ORF1b typing region, A-YHV potentially represents a recombinant between type 1b and type 5. SDS-PAGE and Western blot analysis revealed that type 1b produced a gp116 deletion protein that did not bind with the MAb or polyclonal Ab to normal gp116. Overall, the information suggested that lack of A-YHV virulence was associated with the NS gene sequence linked to ORF1b rather than the deletion in ORF3. | lld:pubmed |
| pubmed-article:19073334 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19073334 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19073334 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19073334 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19073334 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19073334 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19073334 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:19073334 | pubmed:issn | 1096-0341 | lld:pubmed |
| pubmed-article:19073334 | pubmed:author | pubmed-author:Sritunyalucks... | lld:pubmed |
| pubmed-article:19073334 | pubmed:author | pubmed-author:FlegelTimothy... | lld:pubmed |
| pubmed-article:19073334 | pubmed:author | pubmed-author:Sriurairatana... | lld:pubmed |
| pubmed-article:19073334 | pubmed:author | pubmed-author:GangnonngiwWa... | lld:pubmed |
| pubmed-article:19073334 | pubmed:author | pubmed-author:Sang-oumWiwat... | lld:pubmed |
| pubmed-article:19073334 | pubmed:author | pubmed-author:Anantasomboon... | lld:pubmed |
| pubmed-article:19073334 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19073334 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:19073334 | pubmed:volume | 385 | lld:pubmed |
| pubmed-article:19073334 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19073334 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19073334 | pubmed:pagination | 161-8 | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:meshHeading | pubmed-meshheading:19073334... | lld:pubmed |
| pubmed-article:19073334 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19073334 | pubmed:articleTitle | Non-virulence of a recombinant shrimp nidovirus is associated with its non structural gene sequence and not a large structural gene deletion. | lld:pubmed |
| pubmed-article:19073334 | pubmed:affiliation | National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Klong Luang Pratumthani, Thailand. | lld:pubmed |
| pubmed-article:19073334 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19073334 | lld:pubmed |
