| pubmed-article:1906377 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C0015576 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C0680022 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C0002475 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C0312418 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C2257300 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C1707329 | lld:lifeskim |
| pubmed-article:1906377 | lifeskim:mentions | umls-concept:C0036667 | lld:lifeskim |
| pubmed-article:1906377 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:1906377 | pubmed:dateCreated | 1991-8-20 | lld:pubmed |
| pubmed-article:1906377 | pubmed:abstractText | Non-transformed skin fibroblasts derived from five members of a cancer-prone family and three unrelated healthy volunteers were assayed for their levels of activity of the quinone reductase DT-diaphorase and for their sensitivity to the antitumor quinone mitomycin C (MMC). Previous studies of skin fibroblasts derived from one afflicted member of this family (3437T) demonstrated increased resistance to MMC under aerobic exposure conditions and a reduced level of DT-diaphorase. In the present study 3437T cells and a cell strain derived from another afflicted member of the cancer-prone family were found to be hyperresistant to the cytotoxic effects of MMC, and demonstrated negligible DT-diaphorase activity (30 +/- 10 nmol/min/mg protein). Cell strains derived from the three other family members demonstrated intermediate DT-diaphorase activity (400-800 nmol/min/mg protein). Enzyme activities of 1800-6000 nmol/min/mg protein were measured in the three control cell strains. A protein that was reactive with a rabbit polyclonal antibody raised against rat DT-diaphorase and corresponded to the known mol. wt of DT-diaphorase was clearly evident in the three control cell strains, but absent in the two MMC-hyperresistant cell strains. This protein was present in intermediate amounts in the remaining members of the cancer-prone family. Southern analysis of DNA isolated from all eight cell strains and restricted with EcoRI demonstrated the presence of a DNA sequence of approximately 15 kb which hybridized to a rat DT-diaphorase cDNA probe. Northern analysis revealed the presence of an RNA species approximately 1200 bp in size, consistent with that for a human DT-diaphorase mRNA, in all cell strains derived from family members. A post-transcriptional defect would, therefore, appear to be responsible for the decreased enzyme activity observed in the resistant cell strains. These results suggest a role for DT-diaphorase in MMC bioactivation and that reduced levels of the protein may be causally related to the cancer-prone tendency of this family. | lld:pubmed |
| pubmed-article:1906377 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1906377 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1906377 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1906377 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1906377 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1906377 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1906377 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1906377 | pubmed:issn | 0143-3334 | lld:pubmed |
| pubmed-article:1906377 | pubmed:author | pubmed-author:RauthA MAM | lld:pubmed |
| pubmed-article:1906377 | pubmed:author | pubmed-author:PatersonM CMC | lld:pubmed |
| pubmed-article:1906377 | pubmed:author | pubmed-author:MarshallR SRS | lld:pubmed |
| pubmed-article:1906377 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1906377 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:1906377 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1906377 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1906377 | pubmed:pagination | 1175-80 | lld:pubmed |
| pubmed-article:1906377 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:1906377 | pubmed:meshHeading | pubmed-meshheading:1906377-... | lld:pubmed |
| pubmed-article:1906377 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1906377 | pubmed:articleTitle | DT-diaphorase activity and mitomycin C sensitivity in non-transformed cell strains derived from members of a cancer-prone family. | lld:pubmed |
| pubmed-article:1906377 | pubmed:affiliation | Department of Medical Biophysics, University of Toronto, Ontario, Canada. | lld:pubmed |
| pubmed-article:1906377 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1906377 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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