| pubmed-article:19061333 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C0028778 | lld:lifeskim |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C1099354 | lld:lifeskim |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C0011209 | lld:lifeskim |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C0444669 | lld:lifeskim |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C0025938 | lld:lifeskim |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:19061333 | lifeskim:mentions | umls-concept:C0596383 | lld:lifeskim |
| pubmed-article:19061333 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19061333 | pubmed:dateCreated | 2009-1-12 | lld:pubmed |
| pubmed-article:19061333 | pubmed:abstractText | A core-shell-type polyion complex (PIC) micelle with a disulfide cross-linked core was prepared through the assembly of iminothiolane-modified poly(ethylene glycol)-block-poly(L-lysine) [PEG-b-(PLL-IM)] and siRNA at a characteristic optimum mixing ratio. The PIC micelles showed a spherical shape of approximately 60 nm in diameter with a narrow distribution. The micellar structure was maintained at physiological ionic strength but was disrupted under reductive conditions because of the cleavage of disulfide cross-links, which is desirable for siRNA release in the intracellular reductive environment. Importantly, environment-responsive PIC micelles achieved 100-fold higher siRNA transfection efficacy compared with non-cross-linked PICs prepared from PEG-b-poly(L-lysine), which were not stable at physiological ionic strength. PICs formed with PEG-b-(PLL-IM) at nonoptimum ratios did not assemble into micellar structure and did not achieve gene silencing following siRNA transfection. These findings show the feasibility of core cross-linked PIC micelles as carriers for therapeutic siRNA and show that stable micellar structure is critical for effective siRNA delivery into target cells. | lld:pubmed |
| pubmed-article:19061333 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19061333 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19061333 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19061333 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19061333 | pubmed:issn | 1526-4602 | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:IshiiAtsushiA | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:NishiyamaNobu... | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:MatsumotoSato... | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:KataokaKazuno... | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:KoyamaHiroyuk... | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:ObaMakotoM | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:YamasakiYuich... | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:MiyataKanjiro... | lld:pubmed |
| pubmed-article:19061333 | pubmed:author | pubmed-author:ChristieR... | lld:pubmed |
| pubmed-article:19061333 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19061333 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:19061333 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:19061333 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19061333 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19061333 | pubmed:pagination | 119-27 | lld:pubmed |
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| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:meshHeading | pubmed-meshheading:19061333... | lld:pubmed |
| pubmed-article:19061333 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19061333 | pubmed:articleTitle | Environment-responsive block copolymer micelles with a disulfide cross-linked core for enhanced siRNA delivery. | lld:pubmed |
| pubmed-article:19061333 | pubmed:affiliation | Department of Materials Engineering, Graduate School of Engineering, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. | lld:pubmed |
| pubmed-article:19061333 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19061333 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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