Linked Life Data

19026759

Source:http://linkedlifedata.com/resource/pubmed/id/19026759

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pubmed-article:19026759pubmed:abstractTextLong term cell cultures could be obtained from brains of adult sea bass (Dicentrarchus labrax) up to 5 days post mortem. On three different occasions, sea bass brain tissues were dissected, dispersed and cultured in Leibovitz's L-15 media supplemented with 10% fetal bovine serum. The resulting cellular preparations could be passaged within 2 or 3 weeks of growth. The neural cells derived from the first trial (SBB-W1) have now been passaged over 24 times within two years. These cells have been cryopreserved and thawed successfully. SBB-W1 cells are slow growing with doubling times requiring at least 7 days at 22 degrees C. These long term cell cultures could be grown in suspension as neurospheres that were immunopositive for nestin, a marker for neural stem cells, or grown as adherent monolayers displaying both glial and neural morphologies. Immunostaining with anti-glial fibrillary acidic protein (a glial marker) and anti-neurofilament (a neuronal marker), yielded positive staining in most cells, suggesting their possible identity as neural stem cells. Furthermore, Sox 2, a marker for neural stem cells, could be detected from these cell extracts as well as proliferating cell nuclear antigen, a marker for proliferating cells. SBB-W1 could be transfected using pEGFP-N1 indicating their viability and suitability as convenient models for neurophysiological or neurotoxicological studies.lld:pubmed
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pubmed-article:19026759pubmed:articleTitleEstablishment of long term cultures of neural stem cells from adult sea bass, Dicentrarchus labrax.lld:pubmed
pubmed-article:19026759pubmed:affiliationDepartment of Biology, Wilfrid Laurier University, Waterloo, ON, Canada.lld:pubmed
pubmed-article:19026759pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19026759pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed