| pubmed-article:19013454 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0208355 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0008551 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C1980032 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:19013454 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:19013454 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19013454 | pubmed:dateCreated | 2008-12-29 | lld:pubmed |
| pubmed-article:19013454 | pubmed:abstractText | The proteasome is the primary subcellular organelle responsible for protein degradation. It is a dynamic assemblage of 34 core subunits and many differentially expressed, transiently interacting, modulatory proteins. This paper describes a novel affinity chromatography method for the purification of functional human holoproteasome complexes using mild conditions. Human proteasomes purified by this simple procedure maintained the ability to proteolytically process synthetic peptide substrates and degrade ubiquitinated parkin. Furthermore, the entire purification fraction was analyzed by mass spectrometry in order to identify proteasomal proteins and putative proteasome-interacting proteins. The mild purification conditions maintained transient physical interactions between holoproteasomes and a number of known modulatory proteins. In addition, several classes of putative interacting proteins co-purified with the proteasomes, including proteins with a role in the ubiquitin proteasome system for protein degradation or DNA repair. These results demonstrate the efficacy of using this affinity purification strategy for isolating functional human proteasomes and identifying proteins that may physically interact with human proteasomes. | lld:pubmed |
| pubmed-article:19013454 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19013454 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19013454 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19013454 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19013454 | pubmed:issn | 1090-2422 | lld:pubmed |
| pubmed-article:19013454 | pubmed:author | pubmed-author:FonEdward AEA | lld:pubmed |
| pubmed-article:19013454 | pubmed:author | pubmed-author:GottliebBruce... | lld:pubmed |
| pubmed-article:19013454 | pubmed:author | pubmed-author:BeitelLenore... | lld:pubmed |
| pubmed-article:19013454 | pubmed:author | pubmed-author:TrifiroMark... | lld:pubmed |
| pubmed-article:19013454 | pubmed:author | pubmed-author:ScanlonThomas... | lld:pubmed |
| pubmed-article:19013454 | pubmed:author | pubmed-author:DurcanThomas... | lld:pubmed |
| pubmed-article:19013454 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19013454 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:19013454 | pubmed:volume | 315 | lld:pubmed |
| pubmed-article:19013454 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19013454 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19013454 | pubmed:pagination | 176-89 | lld:pubmed |
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| pubmed-article:19013454 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19013454 | pubmed:articleTitle | Isolation of human proteasomes and putative proteasome-interacting proteins using a novel affinity chromatography method. | lld:pubmed |
| pubmed-article:19013454 | pubmed:affiliation | Lady Davis Institute for Medical Research, SMBD-Jewish General Hospital, Montreal, Canada. | lld:pubmed |
| pubmed-article:19013454 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19013454 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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