| pubmed-article:1900915 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C0038423 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C0002245 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1900915 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1900915 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1900915 | pubmed:dateCreated | 1991-4-22 | lld:pubmed |
| pubmed-article:1900915 | pubmed:abstractText | A gene, amy, encoding an alpha-amylase, was cloned on a 4.8 kb Sau3A fragment from the DNA of Streptomyces griseus IMRU3570. The gene was localized to a 2.27 kb fragment by subcloning and deletion mapping experiments. The gene contained an open reading frame (ORF) of 1698 nucleotides that encoded a protein of 566 amino acids with a deduced Mr of 59713 Da. Dot-blot analysis revealed that the copy number of the transcript in S. lividans transformed with the amy gene was 2.8-fold higher than in the donor S. griseus strain in good agreement with the proportionally higher secretion of amylase in S. lividans. A transcription initiation site was found approximately 64 bp upstream from the ATG translation start codon. The promoter of the amy gene was subcloned on a 290 bp HindIII--EcoRI fragment. Expression of a neomycin resistance gene from the amy promoter was negatively regulated by glucose. A 219 nucleotide fragment extending from the single BstEII site to the end of the amy gene was dispensable since active alpha-amylase was secreted after deletion of this region and coupling of a TGA translation stop codon. | lld:pubmed |
| pubmed-article:1900915 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1900915 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1900915 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1900915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1900915 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1900915 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1900915 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:1900915 | pubmed:issn | 0026-8925 | lld:pubmed |
| pubmed-article:1900915 | pubmed:author | pubmed-author:MartínJ FJF | lld:pubmed |
| pubmed-article:1900915 | pubmed:author | pubmed-author:GilJ AJA | lld:pubmed |
| pubmed-article:1900915 | pubmed:author | pubmed-author:DazaAA | lld:pubmed |
| pubmed-article:1900915 | pubmed:author | pubmed-author:VigalTT | lld:pubmed |
| pubmed-article:1900915 | pubmed:author | pubmed-author:García-Gonzál... | lld:pubmed |
| pubmed-article:1900915 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1900915 | pubmed:volume | 225 | lld:pubmed |
| pubmed-article:1900915 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1900915 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1900915 | pubmed:pagination | 278-88 | lld:pubmed |
| pubmed-article:1900915 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:1900915 | pubmed:meshHeading | pubmed-meshheading:1900915-... | lld:pubmed |
| pubmed-article:1900915 | pubmed:meshHeading | pubmed-meshheading:1900915-... | lld:pubmed |
| pubmed-article:1900915 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1900915 | pubmed:articleTitle | Cloning, characterization and expression of an alpha-amylase gene from Streptomyces griseus IMRU3570. | lld:pubmed |
| pubmed-article:1900915 | pubmed:affiliation | Area de Microbiología, Facultad de Biología, Universidad de León, Spain. | lld:pubmed |
| pubmed-article:1900915 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1900915 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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