| pubmed-article:19008397 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0005516 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0376538 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0017393 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0597634 | lld:lifeskim |
| pubmed-article:19008397 | lifeskim:mentions | umls-concept:C0016884 | lld:lifeskim |
| pubmed-article:19008397 | pubmed:issue | Pt 12 | lld:pubmed |
| pubmed-article:19008397 | pubmed:dateCreated | 2008-11-14 | lld:pubmed |
| pubmed-article:19008397 | pubmed:abstractText | Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major problem in the pork industry worldwide. The limitations of current PRRSV vaccines require the development of a new generation of vaccines. One of the key steps in future vaccine development is to include markers for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of type 1 PRRSV, this study constructed a recombinant green fluorescent protein (GFP)-tagged PRRSV containing a deletion of an immunogenic epitope, ES4, in the nsp2 region. In a nursery pig disease model, the recombinant virus was attenuated with a lower level of viraemia in comparison with that of the parental virus. To complement the marker identification, GFP and ES4 epitope-based ELISAs were developed. Pigs immunized with the recombinant virus lacked antibodies directed against the corresponding deleted epitope, but generated a high-level antibody response to GFP by 14 days post-infection. These results demonstrated that this recombinant marker virus, in conjunction with the diagnostic tests, enables serological differentiation between marker virus-infected animals and those infected with the wild-type virus. This rationally designed marker virus will provide a basis for further development of PRRSV marker vaccines to assist with the control of PRRS. | lld:pubmed |
| pubmed-article:19008397 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19008397 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19008397 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19008397 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:19008397 | pubmed:issn | 0022-1317 | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:FangYingY | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:Christopher-H... | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:ChenZhenhaiZ | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:NelsonEricE | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:LawsonSteven... | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:BrownElizabet... | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:LiuHaixiaH | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:KnudsenDavidD | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:ClementTravis... | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:GaoXiaofeiX | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:BreenRachaelR | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:BaoJingjingJ | lld:pubmed |
| pubmed-article:19008397 | pubmed:author | pubmed-author:DalyRussellR | lld:pubmed |
| pubmed-article:19008397 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19008397 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:19008397 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19008397 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19008397 | pubmed:pagination | 3086-96 | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
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| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:meshHeading | pubmed-meshheading:19008397... | lld:pubmed |
| pubmed-article:19008397 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:19008397 | pubmed:articleTitle | Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development. | lld:pubmed |
| pubmed-article:19008397 | pubmed:affiliation | Center for Infectious Disease Research and Vaccinology, Veterinary Science Department, South Dakota State University, Brookings, SD 57007, USA. ying.fang@sdstate.edu | lld:pubmed |
| pubmed-article:19008397 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19008397 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19008397 | lld:pubmed |
