| pubmed-article:19005489 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C0206679 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C2608085 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C1824884 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C0042210 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:19005489 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:19005489 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:19005489 | pubmed:dateCreated | 2009-4-16 | lld:pubmed |
| pubmed-article:19005489 | pubmed:abstractText | Using the T-REx (Invitrogen, Carlsbad, CA) gene switch technology, we previously generated a dominant-negative herpes simplex virus (HSV)-1 recombinant, CJ83193, capable of inhibiting its own replication as well as that of wild-type HSV-1 and HSV-2. It has been further demonstrated that CJ83193 is an effective vaccine against HSV-1 infection in a mouse ocular model. To ensure its safety and augment its efficacy, we generated an improved CJ83193-like HSV-1 recombinant, CJ9-gD, which contains a deletion in an HSV-1 essential gene and encodes an extra copy of gene-encoding glycoprotein D (gD) driven by the tetO-bearing human cytomegalovirus major immediate-early promoter. Unlike CJ83193, which exhibits limited plaque-forming capability in Vero cells and expresses little gD in infected cells, CJ9-gD is completely replication defective, yields high-level expression of gD following infection, and cannot establish detectable infection in mouse trigeminal ganglia following intranasal and ocular inoculation. Mice immunized with CJ9-gD produced 3.5-fold higher HSV-1 neutralizing antibody titer than CJ83193-immunized mice, and were completely protected from herpetic ocular disease following corneal challenge with wild-type HSV-1. Moreover, immunization of mice with CJ9-gD elicited a strong HSV-1-specific T-cell response and led to an 80% reduction in latent infection by challenge wild-type HSV-1 compared with the mock-immunized control. | lld:pubmed |
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| pubmed-article:19005489 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19005489 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19005489 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19005489 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19005489 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19005489 | pubmed:month | May | lld:pubmed |
| pubmed-article:19005489 | pubmed:issn | 1523-1747 | lld:pubmed |
| pubmed-article:19005489 | pubmed:author | pubmed-author:YaoFengF | lld:pubmed |
| pubmed-article:19005489 | pubmed:author | pubmed-author:LuZhemingZ | lld:pubmed |
| pubmed-article:19005489 | pubmed:author | pubmed-author:BransRichardR | lld:pubmed |
