| pubmed-article:1898364 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1898364 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:1898364 | lifeskim:mentions | umls-concept:C0017687 | lld:lifeskim |
| pubmed-article:1898364 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:1898364 | lifeskim:mentions | umls-concept:C0597304 | lld:lifeskim |
| pubmed-article:1898364 | lifeskim:mentions | umls-concept:C0596292 | lld:lifeskim |
| pubmed-article:1898364 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:1898364 | pubmed:dateCreated | 1991-10-23 | lld:pubmed |
| pubmed-article:1898364 | pubmed:abstractText | The effects of insulin and glucagon on liver cell volume and proteolysis were studied in isolated perfused rat liver. The rate of proteolysis was assessed as [3H]leucine release from single-pass-perfused livers from rats which had been prelabelled in vivo by intraperitoneal injection of [3H]leucine. The intracellular water space was determined from the wash-out profiles of simultaneously added [3H]inulin and [14C]urea. In normo-osmotic (305 mosM) control perfusions the intracellular water space was 548 +/- 10 microliters/g wet mass (n = 44) and was increased by 16.5 +/- 2.6% (n = 6), i.e. by 85 +/- 14 microliters/g, after hypoosmotic exposure (225 mosM). Glucagon (0.1 microM) decreased the intracellular water space by 17 +/- 4% (n = 4), whereas insulin (35 nM) increased the intracellular water space by 9.3 +/- 1.4% (n = 15). Also, in isolated rat hepatocyte suspensions insulin (100 nM) caused cell swelling by 10.7 +/- 1.8% (n = 16), which was fully reversed by glucagon. In perfused liver, insulin-induced cell swelling was accompanied by a hepatic net K+ uptake (4.5 +/- 0.2 mumol/g) and an inhibition of proteolysis by 21 +/- 2% (n = 12); further addition of glucagon led to a net K+ release of 3.8 +/- 0.2 mumol/g (n = 7) and fully reversed the insulin effects on both cell volume and proteolysis. Similarly, insulin-induced cell swelling and inhibition of proteolysis were completely antagonized by hyperosmotic (385 mosM) cell shrinkage. Furthermore, cell swelling and inhibition of proteolysis after hypo-osmotic exposure or amino acid addition were reversed by glucagon-induced cell shrinkage. There was a close relationship between the extent of cell swelling and the inhibition of proteolysis, regardless of whether cell volume was modified by insulin, glucagon or aniso-osmotic exposure. The data show that glucagon and insulin are potent modulators of liver cell volume, at least in part by alterations of cellular K+ balance, and that their opposing effects on hepatic proteolysis can largely be explained by opposing effects on cell volume. It is hypothesized that hormone-induced alterations of cell volume may represent an important, not yet recognized, mechanism mediating hormonal effects on metabolism. | lld:pubmed |
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| pubmed-article:1898364 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1898364 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1898364 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1898364 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1898364 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1898364 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:1898364 | pubmed:author | pubmed-author:GerokWW | lld:pubmed |
| pubmed-article:1898364 | pubmed:author | pubmed-author:LaneDD | lld:pubmed |
| pubmed-article:1898364 | pubmed:author | pubmed-author:HäussingerDD | lld:pubmed |
| pubmed-article:1898364 | pubmed:author | pubmed-author:HallbruckerCC | lld:pubmed |
| pubmed-article:1898364 | pubmed:author | pubmed-author:Vom DahlSS | lld:pubmed |
| pubmed-article:1898364 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1898364 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1898364 | pubmed:volume | 278 ( Pt 3) | lld:pubmed |
| pubmed-article:1898364 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1898364 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1898364 | pubmed:pagination | 771-7 | lld:pubmed |
| pubmed-article:1898364 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:1898364 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1898364 | pubmed:articleTitle | Regulation of liver cell volume and proteolysis by glucagon and insulin. | lld:pubmed |
| pubmed-article:1898364 | pubmed:affiliation | Medizinische Universitätsklinik, Freiburg, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:1898364 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1898364 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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