| pubmed-article:18977782 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18977782 | lifeskim:mentions | umls-concept:C0035366 | lld:lifeskim |
| pubmed-article:18977782 | lifeskim:mentions | umls-concept:C0043457 | lld:lifeskim |
| pubmed-article:18977782 | lifeskim:mentions | umls-concept:C0017428 | lld:lifeskim |
| pubmed-article:18977782 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:18977782 | lifeskim:mentions | umls-concept:C0336791 | lld:lifeskim |
| pubmed-article:18977782 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:18977782 | pubmed:dateCreated | 2008-12-25 | lld:pubmed |
| pubmed-article:18977782 | pubmed:abstractText | We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms. | lld:pubmed |
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| pubmed-article:18977782 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18977782 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18977782 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18977782 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18977782 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18977782 | pubmed:issn | 1477-4062 | lld:pubmed |
| pubmed-article:18977782 | pubmed:author | pubmed-author:ChenWenbiaoW | lld:pubmed |
| pubmed-article:18977782 | pubmed:author | pubmed-author:BurgessShawn... | lld:pubmed |
| pubmed-article:18977782 | pubmed:author | pubmed-author:JaoLi-EnLE | lld:pubmed |
| pubmed-article:18977782 | pubmed:author | pubmed-author:MaddisonLiset... | lld:pubmed |
| pubmed-article:18977782 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18977782 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:18977782 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18977782 | pubmed:authorsComplete | Y | lld:pubmed |
