| pubmed-article:18942097 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0005532 | lld:lifeskim |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:18942097 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:18942097 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18942097 | pubmed:dateCreated | 2009-1-28 | lld:pubmed |
| pubmed-article:18942097 | pubmed:abstractText | To develop a sensitive and inducible system to study intestinal biology, we generated a transgenic mouse model expressing the reverse tetracycline transactivator rtTA2-M2 under control of the 12.4 kb murine Villin promoter. The newly generated Villin-rtTA2-M2 mice were then bred with the previously developed tetO-HIST1H2BJ/GFP model to assess inducibility and tissue-specificity. Expression of the histone H2B-GFP fusion protein was observed exclusively upon doxycycline induction and was uniformly distributed throughout the intestinal epithelium. The Villin-rtTA2-M2 was also found to drive transgene expression in the developing mouse intestine. Furthermore, we could detect transgene expression in the proximal tubules of the kidney and in a population of alleged gastric progenitor cells. By administering different concentrations of doxycycline, we show that the Villin-rtTA2-M2 system drives transgene expression in a dosage-dependent fashion. Thus, we have generated a novel doxycycline-inducible mouse model, providing a valuable tool to study the effect of different gene dosages on intestinal physiology and pathology. | lld:pubmed |
| pubmed-article:18942097 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18942097 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18942097 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18942097 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18942097 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18942097 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:18942097 | pubmed:issn | 1526-968X | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:KuipersErnst... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:FoddeRiccardo... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:SmitsRonR | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:FrankenPatric... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:RottierRobber... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:BeverlooBerna... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:BlondenLauL | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:RothSabrinaS | lld:pubmed |
| pubmed-article:18942097 | pubmed:author | pubmed-author:RaghoebirLali... | lld:pubmed |
| pubmed-article:18942097 | pubmed:copyrightInfo | Copyright 2008 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:18942097 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18942097 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:18942097 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18942097 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18942097 | pubmed:pagination | 7-13 | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
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| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:meshHeading | pubmed-meshheading:18942097... | lld:pubmed |
| pubmed-article:18942097 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:18942097 | pubmed:articleTitle | Generation of a tightly regulated doxycycline-inducible model for studying mouse intestinal biology. | lld:pubmed |
| pubmed-article:18942097 | pubmed:affiliation | Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands. | lld:pubmed |
| pubmed-article:18942097 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18942097 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18942097 | lld:pubmed |
